脐血CD3AK细胞和其培养上清诱导K562细胞凋亡

Cord blood CD3AK cells and CD3AK cell cultural supermatants induce K562 cell apoptosis

摘要:

目的 探讨脐血CD3 AK细胞和其培养上清 (CS)诱导K562 细胞凋亡。方法 用细胞形态学观察 ,DNA琼脂糖电泳 ,原位末端标记法检测K562 ,CD3 AK细胞诱导的K562 ,CS诱导的K562 细胞凋亡率。结果 K562 细胞自然凋亡率 3.5 0± 0 .97% ;CD3 AK细胞诱导的K562 细胞凋亡率 2 7.38± 4.91% ,P <0 .0 1,CS诱导的K562 细胞凋亡率为 33.0 9± 5 .2 2 % ,P <0 .0 1。结论 脐血CD3 AK细胞和其培养上清能诱导K562 凋亡。

Abstract:

Objective To explore K 562 cell apoptosis induced by cord blood CD 3AK cells and CD 3AK cell cultural supernatants (CS). Methods Apoptosis was detected in K 562 cells and in K 562 cells cultured with either cord blood CD 3AK cells or CS using the TDT end labelling technique, cell morphology and DNA agarose gel electrophoresis. Results The percentage of apoptotic cells in the control K 562 culture was 3.50 ± 0.97% . Apoptosis of K 562 cells cultured with CD 3AK cells rose to 27.38 ± 4.91% (P< 0.01 ). Culturing K 562 cells in CS also resulted in significantly increased apoptosis ( 33.09 ± 5.22% ) (P< 0.01 ). Conclusions Cord blood CD 3AK cells and CS could induce K 562 cell apoptosis. [