CMV和HCV肝炎婴儿血清IFN-α,IL-8,TNF-α和NO的水平变化

Levels of Serum IFN-α, IL-8, TNF-α and NO in the CMV and HCV Hepatitis in Infants

摘要:

目的 了解巨细胞病毒肝炎 (CMV IgM抗体阳性 )和丙型肝炎 (HCV IgM抗体阳性 ) 1～ 6月的婴儿患者血清中某些炎性介质的异常变化。方法 应用ELISA法和硝酸还原酶法 ,分别检测了 5 6例CMV抗体和6 7例HCV抗体阳性婴儿肝炎综合征 (婴肝 )患儿及正常对照组 5 8例小儿的血清干扰素α (IFN α) ,白细胞介素 8(IL 8) ,肿瘤坏死因子 α (TNF α)和一氧化氮 (NO)的含量。结果 正常对照组 5 8例血清IFN α ,IL 8,TNF α ,NO含量分别为 2 2 6 .74± 73.82ng/L ,13.2 4± 5 .36ng/L ,2 17.14± 76 .30ng/L ,2 5 .98± 8.70 μmol/L。CMV抗体阳性婴肝组血清IFN α ,IL 8,TNF α和NO的含量分别为 5 82 .2 6± 131.72ng/L ,75 .2 8± 33.5 7ng/L ,42 9.46± 15 6 .32ng/L和5 9.87± 16 .42 μmol/L ,明显高于正常对照组 (P <0 .0 1) ;HCV抗体阳性婴肝组上述四项指标含量分别为5 5 8.32± 114.6 4ng/L ,71.34± 2 7.6 4ng/L ,374.35± 138.4ng/L和 6 2 .2 4± 2 1.38μmol/L ,其含量亦明显高于正常对照组 (P <0 .0 0 1)。CMV与HCV抗体阳性婴肝组比较 ,无明显差异 (P >0 .0 5 )。TNF α和NO在这两组婴肝中分别呈正相关 (r1=0 .6 2 ,r2 =0 .5 7,P<0 .0 1)。结论 CMV和HCV抗体阳性婴肝急性期患儿 ,血清TNF α,IL 8,IFN

Abstract:

Objective To study variations of inflammatory mediators in the serum of children aged 1～6 months with infantile hepatitis. Methods Using ELISA and carbonate reductase assays, we measured the levels of serum IFN α, IL 8, TNF α and NO in 56 children suffering from CMV antibody positive infantile hepatitis, 67 children suffering from HCV antibody positive infantile hepatitis and 58 normal children. Results In the normal control group, the levels of INF α, IL 8 and TNF α were226.74±73.82 ng/L, 13.24±5.36 ng/L, 217.14±76.30 ng/L, respectively, and NO concentrations were 25.98±8.70 μmol/L. In the CMV antibody postive group, IFN α Levels were 582.26 ± 131.72 ng/L , IL 8 levels were 75.28 ± 33.57 ng/L, TNF α levels were 429.46 ± 156.32 ng/L, and NO concentrations were 59.87 ± 16.42 μmol/L. These values were all significantly higher than those of the normal control group (P< 0.01 ). In the HCV antibody positive group, the concentrations of IFN α, IL 8, TNF α and NO were 558.32 ± 114.64 ng/L , 71.34 ± 27.64 ng/L, 374.35 ± 138.4 ng/L and 62.24 ± 21.38 μmol/L, respectively. These levels were also significantly increased compared to the controls (P< 0.01 ). There were no significant differences between the CMV antibody positive and the HCV antibody positive groups. TNF α and NO were positively correlated in both groups (r 1 = 0.62 , r 2 = 0.57 , P< 0.01 ). Conclusions Serum levels of IFN-α, IL 8, TNF α, and NO increase in the critical period of the infantile hepatitis syndrome with CMV and HCV antibody positive. [