主动脉球囊损伤大鼠血浆内皮素及胰岛素样生长因子-Ⅰ的动态改变

Dynamic Changes of IGF-I and ET in the Development of Restenosis after Balloon Angioplasty in Rats

摘要:

目的 探讨内皮素 (endothelin ,ET)及胰岛素样生长因子 -Ⅰ (insulinlikegrowthfactor-I ,IGF -Ⅰ )在先天性血管畸形球囊成形术后再狭窄中的作用。方法 用自制导管制造大鼠血管内皮损伤模型 ,观察不同时间点受损血管病理改变 ,同时用放射免疫法测定血浆ET及IGF -Ⅰ含量。结果 血管内皮损伤后 ,随着时间延长 ,平滑肌细胞大量增殖造成管腔狭窄 ,而血浆ET及IGF -Ⅰ升高明显 ,各时间点与对照组相比均有显著性差异。且两者具有较好相关性 (r=0 .6 2 ,P <0 .0 5 )。结论 ET与IGF -Ⅰ相互作用促进血管平滑肌增殖 ,两者共同参与了球囊血管成形术后再狭窄的病理过程。

Abstract:

Objective To investigate the effect of endothelin (ET) and insulin like growth factor-Ⅰ (IGF-Ⅰ) on the development of restenosis after balloon angioplasty. Methods Using a catheter to induce the endothelium injury, we created a rat model of aortic endothelium injury. *"Pathological changes of the injuried aortic endothelium were assessed, and the plasma concentration of ET and IGF-Ⅰ were determined with radioimmunoassay. Results As demonstrated by pathological examination, aortic injury related restenosis was induced by the proliferation of vascular smooth muscle cells. Plasma concentrations of ET and IGF-Ⅰ increased significantly, and there was a significant correlation between ET and IGF-Ⅰ at different time points (r= 0.62 , P< 0.05 ). Conclusions ET and IGF-Ⅰ, as stimulators of vascular smooth muscle cell proliferation, might participate in the pathological process of restenosis after balloon angioplasty.