IGFBP-1在胎鼠生长发育中作用的研究

The Role of IGFBP-1 in Fetal Rat Growth

摘要:

目的 探讨胰岛素样生长因子结合蛋白 - 1(IGFBP - 1)在正常胎鼠发育和胎鼠宫内发育迟滞(IUGR)发生中的作用。方法 钳夹孕鼠双侧子宫动、静脉 2 0min ,建立胎鼠IUGR模型 ,观察实验组 (IUGR组 )和正常对照组胎鼠血清IGFBP - 1水平与胎鼠血糖水平、生长参数 (身长、体重 )和器官重量 (胎盘、肺、脑、肝重 )的关系。结果 实验组胎鼠的体重、肺、脑、肝依次为 (1.93± 0 .2 3) g ,(0 .39± 0 .0 1) g ,(0 .10± 0 .0 1) g ,和 (0 .18±0 .0 2 )g ;身长为 (2 .70± 0 .16 )cm ,血糖水平为 (42 .49± 9.2 5 )mg/ml均明显低于对照组的 (3.87± 0 .85 )g ,(0 .47± 0 .0 4) g ,(0 .15± 0 .0 2 ) g ,和 (0 .2 2± 0 .0 6 ) g ,(3.6 1± 0 .44 )cm ,血糖 (6 7.31± 7.12 )mg/ml。血清IGFBP - 1(94.99± 16 .0 7)ng/ml较对照组 (33.2± 6 .96 )ng/ml明显升高 (均P <0 .0 1) ,血清IGFBP - 1与胎鼠血糖水平、生长参数、器官重量呈显著负相关 (均P <0 .0 1)。结论 血清IGFBP - 1作为生长抑制因子参与正常胎鼠发育及IUGR的发生。

Abstract:

Objective To explore the role of IGFBP-1 in the normal growth and intrauterine growth retardation (IUGR) in rat fetuses. Methods The bilateral uterine arteries and veins of SD pregnant rats of the experimental group were clamped for 20 minutes. A IUGR model was set up. A sham operation was performed on the control. We observed the correlation between the serum IGFBP-1 level and blood glucose level, growth parameters (body weight and length) and organ weight (placenta, lung, brain and liver) of the rat fetuses. Results ①The growth parameters, organ weight and blood glucose level ( 42.49 mg/ml) of the experimental group decreased significantly while the serum IGFBP-1 ( 94.99 ng/ml) increased significantly compared with those of the control group (IGFBP-1 33.20 ng/ml blood glucose 67.31 mg/ml) ( P < 0.01 ); ②There was a significant negative correlation between the serum IGFBP-1 level and the blood glucose level, growth parameters and organ weight of all the rat fetuses including IUGR and control groups ( P < 0.01 ). Conclusions Serum IGFBP-1 participates in the growth of normal and IUGR rat fetuses as an inhibiting factor.