年龄依赖性癫痫性脑病患儿的血清细胞因子变化

Changes of Serum IL-2, TNF Alpha and IFN Alpha Levels in Children with Age-dependent Epileptic Encephalopathy

摘要:

目的 探讨白细胞介素2(IL-2)、肿瘤坏死因子α (TNFα)和干扰素α (IFNα)在年龄依赖性癫痫性脑病(ADEE)患儿血清中的变化。方法 采用双抗体夹心酶联免疫吸附试验(ELISA)检测20例ADEE患儿血清中IL-2，TNFα和IFNα的水平，年龄和性别相匹配的20例正常儿童为对照组。结果 癫痫组血清IL-2，TNFα和IFNα水平均明显高于对照组分别为(379.53±44.86) ng/L比(239.66±21.03) ng/L；(962.42±107.69) ng/L比(501.62±38.93) ng/L；(586.12±84.86) ng/L比(329.56±30.43) ng/L。(t=2.59，3.51，2.71，P分别<0.05，0.01，0.05)。相关分析发现ADEE患儿血清IL-2与TNFα水平之间呈显著正相关(r=0.671，P<0.01)。结论 ADEE患儿存在免疫功能异常，细胞因子参与了免疫病理损害过程。

Abstract:

Objective To determine the changes ofserum interleukin-2 (IL-2), tumor necrosis factor alpha (TNF α) and interferon alpha (IFN α) levels in children with age-dependent epileptic encephalopathy (A DEE). Methods We used double antibody sandwiched ELISA to measu re serum IL-2, TNF α and IFN α levels in 20 children with ADEE. Twenty healthy children who were matched by age and sex were in the control group. Res ults The serum IL-2, TNF α and IFN α levels of the epilepsy group wer e statistically higher than those in the control group (379.53±44.86) n g/L vs. (239.66±21.03) ng/L; (962.42±107.69) ng/L vs. (501.6 2±38.93) ng/L; (586.12±84.86) ng/L vs. (329.56±30.43) n g/L; respectively. (t=2.59, 3.51, 2.71,P<0.05, P< 0.01,P<0.05, respectively). The serum level of IL-2 was positively co rrelated with that of TNF α in children with ADEE (r=0.671,P<0.01). Conclusions Children with ADEE had abnormal immunologic f unction. The cytokines might be involved in the pathogenesis of ADEE.