幼兔胎粪吸入性肺损伤后肺组织血栓素B_2 6-酮前列环素1α水平及其意义

Level of TXB2 and 6-keto-PGF1α in the Lung of Immatury Rabbits with Meconium Aspiration after Lung Injury

摘要:

目的了解幼兔胎粪吸入后肺组织中血栓素B2(TXB2)和6-酮前列环素1α (6-keto-PGF-1α)的动态变化。方法将动物分为轻度胎粪吸入组(16 h，24 h，48 h，72 h)、重度胎粪吸入组和对照组，通过气管内灌入胎粪0.6 ml/kg和4 ml/kg建立轻、重度幼兔胎粪吸入模型；应用右心室穿刺法经压力传感器与日本光电公司生产RM-6000型多道生理记录仪相连，测定右心室收缩压；应用放射免疫法检测肺组织中TXB2和6-keto-PGF-1α含量。结果轻度胎粪吸入组右心室收缩压从胎粪吸入后16 h升高(2.57±0.10 kPa)，24 h达高峰(3.57±0.14 kPa)，72 h恢复正常(1.89±0.14 kPa)。重度胎粪吸入组右心室收缩压(4.36±0.14 kPa)明显高于轻度胎粪吸入组和对照组(1.85±0.05 kPa)；轻度幼兔胎粪吸入16 h肺组织TXB2(153.80±15.37 pg/ml)和6-keto-PGF-1α(117.40±22.88 pg/ml)水平开始增高，24 h达高峰(369.00±28.80 pg/ml；207.20±28.59 pg/ml)，至72 h开始降低(103.20±11.95 pg/ml；83.62±8.76 pg/ml)，重度胎粪吸入肺组织TXB2(500.60±24.58 pg/ml)和6-keto-PGF-1α(300.00±20.31 pg/ml)增加更明显。TXB2和6-Keto-PGF1α分别与右心室压作相关分析，均具有明显相关性(r=0.95；0.96, P<0.01)。结论随着胎粪吸入性肺损伤加重，TXB2和6-Keto-PGF1α增加更明显，表明TXB2和6-keto-DGF-1α参与胎粪吸入性肺损伤发生发展。

Abstract:

Objective To study the changes of TXB2 and 6 - keto - PGF1α in the, lung of immature rabbits with meconium aspiration. Methods We established mild and severe immature rabbit models of meconium aspiration by endotracheal intubation imbuing meconium 0. 6 ml/kg and 4 ml/kg. We measured the right ventricular pressure by right ventricular puncture. Levels of TXB2 and 6 - keto - PGF1α in the lung were measured by radioimmunoassay. Results In the mild group, the right ventricular pressure began to increase at 16 hours (2. 57 ± 0. 10 kPa), peaked at 24 hours (3. 57 ± 0. 14 kpa) and returned to the normal level by 72 hours (1. 89 ± 0. 04 kPa). The pressure of the severe group (4. 36 ± 0. 14 kpa)was significantly higher than that of the control (1. 85 ± 0. 05 kPa) and mild groups. Levels of TXB2 and 6 - keto - PGF1α. in the lung of the mild group increased significantly compared with the control group (76. 40 ± 16. 89 pg/ml; 56. 10 ± 16. 46 pg/ml), began to increase at 16 hours (153. 80 ± 15. 37 pg/ml, 117. 40 ± 22. 88 pg/ml),. peaked at 24 hours (369. 00 ± 28. 80 pg/ml; 207. 20 ± 28. 59 pg/ml) and recovered by 72 hours (103. 20 ± 11. 95 pg/ ml, 83. 62 ± 8. 76 pg/ml). The changes of the severe group (500. 60 ± 24. 58 pg/ml; 300. 00 ± 20. 31 pg/ml) were significantly different from those in the control and mild groups. The level of TXB2 and 6 - keto - PGF1. were correlated with the right ventricular pressure (r = 0. 95: 0. 96. P <0. 01). Conclusions TXB2 and 6 - keto -PGF1α in the lung significantly increase following the severity of meconium aspiration, indicating that TXB2 and 6 - keto - PGF1. influence the development of lung injury of meconium aspiration.