成年大鼠骨髓基质细胞体外诱导分化为NSE阳性细胞

Differentiation of Adult Rat Bone Marrow Stromal Cells Into NSE positive Cells

摘要:

目的： 研究成年大鼠骨髓基质细胞 (MSCs)在体外分化为NSE阳性细胞的方法。方法： 采用 β -巯基乙醇预诱导MSCs24h,再诱导5h。神经细胞特异性烯醇化酶 (NSE)免疫细胞化学染色鉴定、计数诱导前后的细胞。结果： 诱导后MSCs形态改变,胞体呈锥形,突起交织成网。免疫细胞化学染色NSE表达率达到 (6 3.7±4.5) %。结论： β -巯基乙醇可以诱导成年大鼠MSCs在体外分化成为NSE阳性细胞。

Abstract:

OBJECTIVE: To study the protocol that induces adult rat bone marrow stromal cells (MSCs) to express neuron specific enolase (NSE) in vitro. METHODS: MSCs were preinduced with β mercaptoethanol for 24 h, and then induced for 5 h. The positive percentages of NSE protein expression were measured by immunocytochemistry SABC staining. RESULTS: After the induction, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and processes which formed extensive networks. The positive percentage of NSE protein expression was ( 63.7 ± 4.5 )%. CONCLUSIONS: β mercaptoethanol may induce adult rat MSCs to NSE positive cells in vitro.