儿童激素敏感性肾病综合征CD30~+细胞及TH2类细胞因子研究

State of CD30+ Cells and TH2 Cytokines in Children with the Steroid Sensitive Nephrotic Syndrome

摘要:

目的:了解糖皮质激素敏感性肾病综合征(SSNS)患儿CD30阳性细胞及分泌白细胞介素4(IL-4)，5，6的TH2细胞活化状态，进一步证实SSNS免疫异常模式。方法:流式细胞仪(FACS)检测SSNS与健康儿童CD30阳性细胞百分率、ELISA检测外周血单个核细胞(PBMC)培养上清中IL-4，5，6及血清IgE浓度；用SSNS患儿CD30细胞百分率与PBMC培养上清中IL-4，5，6及血清IgE作直线相关分析。结果:SSNS患儿CD30阳性细胞率、IL-4及血IgE(11.96% lg2.11 pg/ml，299 IU/ml)显著高于健康儿童(2.81% lg 1.37 pg/ml, 110 IU/ml)(P均<0.01)，但IL-5，6水平与健康儿童比较则未见显著性差异(P>0.05)；SSNS患儿CD30阳性细胞百分率与IL-4及血IgE水平相关有显著性(r=0.94，P<0.01，r=0.74，P<0.01)；与IL-5、IL-6水平无显著性相关(P<0.05)。结论:SSNS患儿CD30阳性细胞增加并不意味着存在TH2类细胞全面活化，SSNS患儿既有某些TH2类细胞因子(IL-4)升高，又有一些TH2细胞因子的降低(IL-5)或无变化(IL-6)，本文资料不能证明SSNS发病与TH1/TH2失衡有直接关联。

Abstract:

Abstract: OBJECTIVE: To study the TH2 state as represented by the percentage of CD30+ cells, levels of IL24, IL25, and IL26 in PBMC, and serum IgE levels in children with the steroid sensitive nephrotic syndrome(SSNS). METHODS: CD30+ cells were assayed using FACS. IL24, 5, 6 and serum IgE were determined using EL ISA. RESULTS: There was a higher percentage of CD30+ cells (11.96%) and higher levels of IL24 (lg2.11 pg/ml) and serum IgE (299 IU/ml) in children with SSNS compared to the healthy control group (2.81%, lg1.37 pg/ml, 110 IU/ml, respectively) (P<0.01). No significant difference was found in the levels of IL25 and IL26 in the two groups. There was a significant correlation between the percentage of CD30+ cells and IL24(r=0.94, P<0.01) and between CD30+ cells and serum IgE(r=0.740 P<0.01). NO correlation was noted with IL25(r=-0.39, P>0.05)or with IL26 levels (r=0.08,P>0.05). CONCLUSIONS: The abnormal increase of CD30+ cells does not mean that all TH2 cells are activated. The TH1/TH2 imbalance in children with SSNS is not proved in this study.