缺氧对鼠脑微血管内皮细胞分泌组织型纤溶酶原激活物的影响

Effect of Hypoxia on the Activity of the Tissue-Type Plasminogen Activator of Brain Microvascular Endothelial Cells in Mice

摘要:

目的：探讨缺氧对体外培养鼠脑微血管内皮细胞分泌组织型纤溶酶原激活物(tissue type plasminogen activator, TPA)的影响。方法：分别对新生小鼠脑微血管内皮细胞进行原代和传代缺氧条件下培养，常规培养作为空白对照，每组各取8例，吸取培养液用酶联免疫吸附试验测试TPA活性。结果：原代培养内皮细胞空白对照组、缺氧组分泌TPA活性分别为(19.4±1.7)×10-2 IU/ml，(22.5±1.5)×10-2 IU/ml，两者有显著性差异(P<0.01)；传代培养内皮细胞缺氧组与空白对照组分泌TPA活性差异无显著性意义(P>0.05)。结论：体外培养鼠脑微血管内皮细胞能合成分泌TPA；缺氧状态下原代培养内皮细胞产生的TPA活性增高。内皮细胞分泌的TPA可能是脑缺氧缺血致不可逆神经元损伤的一个重要媒介。

Abstract:

OBJECTIVE: To observe the effect of hypoxia on the activity of the tissue-type plasminogen activator (TPA) of brain microvascular endothelial cells in mice. METHODS: Microvascular endothelial cells obtained from the brains of newborn mice were cultured in primary and passage under room air and hypoxic conditions to observe the variation in TPA activities. TPA activity was assayed using the enzyme linked immunosorbent assay (ELISA) technique. RESULTS: Following hypoxia, TPA activity in endothelial cells increased in primary culture compared with the controls: (22.5±1.5)×10-2 IU/ml vs (19.4±1.7)×10-2 IU/ml (P<0.01). TPA activity following hypoxia was not altered in passaged cells. CONCLUSIONS: Neuronal damage following hypoxia may be associated with increased endothelial TPA activity.