当归多糖增强放射损伤小鼠胸腺细胞增殖及红细胞C3b受体粘附功能的实验研究

Effect of Angelica Polysaccharide on the Promotion of Thymocyte Proliferation and RBC C3bR Immune Adhesion Function in Radiated Mice

摘要:

目的：进一步探讨当归多糖(Angelica polysaccharide, AP)的药用价值。方法：检测正常对照组、放射对照组及放射+当归多糖治疗组3组的刀豆蛋白A(ConA)诱导的胸腺细胞增殖反应和红细胞C3b受体花环率。结果：放射对照组胸腺细胞OD值和红细胞C3b受体花环率［(0.24±0.01)，(16.46±0.76)%］明显低于正常对照组［(0.35±0.01)，(22.30±1.26)%］，而放射+当归多糖治疗组的胸腺细胞OD值和红细胞C3b受体花环率［(0.31±0.01)，(21.07±1.22%)］显著高于放射对照组［(0.24±0.01)，(16.46±0.76)%］，P<0.01，有统计学意义。结论：当归多糖能明显增强放射损伤小鼠的胸腺细胞增殖和红细胞C3b受体免疫粘附功能。

Abstract:

OBJECTIVE: To explore the effect of angelica polysaccharide (AP) on the thymocyte proliferation and RBC C3bR immune adhesion function in radiated mice. METHODS: Mice were divided into control, irradiated and AP treated also irradiated groups (10 per group). The proliferation of thymocytes and RBC C3bR rosette formation rate were then examined on day 10 after AP was given. RESULTS: The thymocyte proliferation responses (0.24±0.01) and the RBC C3bR rosette formation rate ［(16.46±0.76)%］ of the irradiated control group were significantly lower than those of the non irradiated controls ［(0.35±0.01) and (22.30±1.26)%, respectively; P<0.01］］. The thymocyte proliferation (0.31±0.01) and RBC 36bR rosette formation ［(21.07±1.22)%］ in the treatment group were significantly higher (P<0.01) than those of the irradiated control group, and approached nonirradiated control levels. CONCLUSIONS: AP can reverse radiationinduced reduction in the proliferation response of thymocytes and in RBC immune adhesion function.