呼吸道感染患儿粒细胞集落刺激因子的测定意义

Determination and Significance of Granulocyte Colony Stimulating Factors in Children with Respiratory Airway Infection

摘要:

目的：探讨粒细胞集落刺激因子(G-CSF)在呼吸道感染个体中的变化。方法：采用双抗夹心酶联免疫法和免疫单扩法对189例呼吸道感染急性期及50例治疗后5～7 d患儿和30例健康儿童进行血G-CSF和C反应蛋白(CRP)检测，同步外周血白细胞计数。结果：血G-CSF阳性105/189例(55.56%)，CRP阳性68/189例(35.98%)，同一病人G-CSF和CRP重叠阳性45/189例(23.81%)。治疗后5～7 d复查50例CRP全部转阴，G-CSF 16/50例(32%)持续阳性。结论：G-CSF水平在急性感染时增高，对临床有一定指导意义。

Abstract:

OBJECTIVE: To explore changes of the serum granulocyte colony-stimulating factor (G-CSF) in children with acute respiratory airway infection. METHODS: Venous blood was obtained from 30 normal children, 189 children with acute respiratory airway infection without treatment and 50 children (from the group of 189 children) treated with antibiotics for 5～7 days. G-CSF levels were determined using the double antibody sandwich enzymelinked immunosorbent assay (PAS ELISA) and C reaction protein (CRP) levels were assayed using singleradial immunodiffusion. RESULTS: Of the 189 patients with acute respiratory airway infection, 105 were positive for G-CSF and 68 were positive for CRP (>12 ug/ml). Both G-CSF and CRP were positive in 23.8% of the patients. After a 5～7 day treatment course, the CRP of 50 turned from positive to negative and the G-CSF of 16 remained positive. CONCLUSIONS: Serum G-CSF levels increase in the stage of acute infection.