Phadiatop和sIgE检测在儿童哮喘诊断中的应用

Application of Phadiatop Test and Specific IgE Test in theDiagnosis of Children with Asthma

摘要:

目的：探讨CAP过敏原检测系统中吸入性过敏原过筛试验(Phadiatop)检测和特异性IgE(sIgE)检测在小儿哮喘病因诊断中的价值。方法：应用荧光酶联免疫法，对153例小儿哮喘、40例支气管肺炎进行Phadiatop，sIgE和血清总IgE检测。结果：哮喘患儿Phadiatop和尘螨sIgE阳性率分别为 66.01%和59.48%，而支气管肺炎患儿两者阳性率分别为 12.5%和7.5%，两组间差异有显著性(P<0.05)。Phadiatop,sIgE灵敏度分别为0.66和0.59，特异度分别为0.88和0.93，两种检测的一致性高。结论：Phadiatop检测是哮喘病因诊断简便、可靠的体外过筛试验，Phadiatop阳性患儿应进一步作sIgE检测。

Abstract:

OBJECTIVE: To explore the value of Phadiatop test and specific IgE test in the diagnosis of children with asthma. METHODS: Using Flo ELISA, Phadiatop, specific IgE and total IgE of serum were determined in 153 cases with asthma and 40 cases with bronchopneumonia. RESULTS: The positive rates of Phadiatop and specific IgE in the asthma group (66.01% and 59.48%, respectively) were significantly higher than those in the bronchopneumonia group (12.5% and 7.5%, respectively)(P<0.05). Phadiatop test had a sensitivity of 0.66 and a specificity of 0.88, and sIgE test to dust mite had a sensitivity of 0.59 and a specificity of 0.93 respectively. There was a concordant result of 94% between the two tests. CONCLUSIONS: Phadiatop test is a useful screening test for the diagnosis of asthma. sIgE test is further needed for the Phadiatop test positive cases.