野百合碱诱导大鼠肺高压时韧粘素mRNA表达水平的动态变化

Levels of Tenascin mRNA in Monocrataline Induced Pulmonary Hypertension in Rats

摘要:

目的：观察野百合碱(MCT)所致肺高压大鼠肺组织和肺动脉中韧粘素(TN)mRNA的动态表达水平，探讨其与肺高压肺血管重建的关系。方法：SD大鼠随机分为MCT组和正常对照组(CON)，采用野百合碱皮下注射法诱导建立大鼠肺高压模型，并通过快速竞争性RTPCR技术检测不同时间点(给药后第7，14，21，28天)大鼠肺组织和肺动脉中TNmRNA的表达水平。结果：TN mRNA水平在给药后第7天即有上调，CON组肺组织TNmRNA为0.29±0.04，MCT组为0.56±0.08，两组间差异有显著性(P<0.01)；CON组肺动脉TN mRNA 为0.30±0.04，MCT组为0.57±0.05，两组间差异有显著性(P<0.05)。TN mRNA水平上调早于肺动脉压的升高，随压力上升和时间延长TN mRNA继续增高。结论：TN参与了肺高压肺血管重建过程，在肺高压发病中可能具有重要作用。

Abstract:

OBJECTIVE: To study the levels of Tenascin (TN) mRNA in pulmonary vascular remodeling associated with pulmonary hypertension(PH) induced by monocrataline (MCT). METHODS: SD rats were randomly assigned into the control (CON) group and MCT group. The model of PH was created by injecting hypodermatic MCT. Rapid competitive RT PCR was used to examine the levels of TN mRNA in central pulmonary arteries (PAs) and lungs at different times after MCT or NS injections. RESULTS: Increased expression of TN mRNA was detected 7 days after the injection of MCT. The expression of TN mRNA in lungs of the MCT group (0.56±0.08) was higher than that of the CON group (0.29±0.04)，P<0.01. TN mRNA in PAs of the MCT group (0.57±0.05) was higher than that of the CON group (0.30±0.04)，P<0.05. The increase of the level of TN mRNA preceded the elevation of pulmonary pressure (14 days) and continued to increase with the rising of pulmonary pressure. CONCLUSIONS: TN might be involved in the pathogenesis of pulmonary vascular remodeling with PH.