儿童急性淋巴细胞白血病DNA倍性与体内白血病细胞凋亡

DNA Ploidy and Apoptosis in Vivo in Childhood Acute Lymphoblastic Leukemia

摘要:

目的：探讨儿童急性淋巴细胞白血病(急淋)肿瘤细胞DNA倍性与凋亡间的关系。方法：检测22例初治急淋患儿的DNA倍性及化疗前后体内白血病细胞的凋亡情况。结果：化疗前所有患儿体内白血病细胞均无明显凋亡，化疗后DNA指数为 1.16～1.6 的高二倍体组凋亡细胞比例为(22.06±8.98)%，较非高二倍体组(9.38±10.27)%显著升高(P<0.01)。结论：儿童高二倍体急性淋巴细胞白血病预后优于其它DNA倍性的病人与此类白血病细胞易凋亡、对化疗敏感有关。

Abstract:

OBJECTIVE: To explore the relationship between DNA ploidy and apoptosis in vivo in childhood acute lymphoblastic leukemia (ALL). METHODS: Using flow cytometry, the assay of apoptosis of bone marrow lymphoblasts of 22 children with recent onset ALL before and after chemotherapy was done. RESULTS: No apoptotic cell was found in any of the cases before chemotherapy. During chemotherapy, the percentage of apoptotic cells in hyperdiploid cases with DNA index (DI) 1.16 ～ 1.6 ( 22.06% ± 8.98% ) was higher compared with that of non hyperdiploid cases with DI< 1.16 or DI> 1.6 ( 9.38% ± 10.27% )(P< 0.01 ).CONCLUSIONS: Hyperdiploid lymphoblasts are easier to undergo apoptosis in vivo, which is responsible for a good prognosis in childhood hyperdiploid ALL.