病毒性心肌炎小鼠心肌细胞凋亡和坏死的研究

Apoptosis and Necrosis of Myocardial Cells in Mice with Viral Myocarditis

摘要:

目的： 探讨病毒性心肌炎小鼠心肌细胞的凋亡与坏死之间的相互关系。方法： 用4周龄Balb/c雄性小鼠腹腔接种柯萨奇B3病毒 (CVB3)制成病毒性心肌炎小鼠模型 ,采用膜联蛋白V联合PI染色双参数技术(AnnexinV/PI)在流式细胞仪上定量检测心肌细胞的凋亡和坏死。结果： 病毒性心肌炎小鼠心肌细胞的凋亡百分率和坏死百分率均显著高于正常对照小鼠(P<0.01),并且心肌细胞凋亡百分率及坏死百分率均与心肌病理积分呈正相关(r =0.70 ,P<0.01和r=0.93,P<0.01)。结论： 病毒性心肌炎小鼠同时存在着心肌细胞的凋亡和坏死 ,两者均与心肌病理损害程度密切相关,为病毒性心肌炎的重要病理过程

Abstract:

OBJECTIVE: To investigate the relationship between apoptosis and necrosis of myocardial cells in murine myocarditis. METHODS: Four week old male Balb/c mice were inoculated with Coxsackievirus B 3 (CVB 3) intraperitoneally as myocarditis models. Annexin V/PI and flow cytometry were used to detect quantitatively the myocardial cell apoptosis and necrosis. RESULTS: The percentage of myocardial cell apoptosis and necrosis in mice with viral myocarditis was much higher than that in normal controls (P<0.01). Both the percentages of apoptosis and necrosis in myocardial cells were correlated positively with the myocardial histopathological score (r= 0.70 and r= 0.93 , respectively, P<0.01). CONCLUSIONS: There are cell apoptosis and necrosis simultaneously in myocardial cells of viral myocarditis. Both the myocardial cell apoptosis and necrosis are closely correlated with the severity of cardiopathological alterations.