小儿哮喘与肺炎支原体肺炎血中一氧化氮和氧自由基的不同变化及意义

Changes and Significance of Serum Nitric Oxide and Oxygen Free Radical in Children with Asthma or Mycoplasma Pneumonia

摘要:

目的： 探讨一氧化氮(NO)、脂质过氧化物(LPO)、血栓素B2 (TXB2 )、循环内皮细胞(CEC)在小儿哮喘及肺炎支原体(MP)肺炎中的作用。方法： 分别检测36例小儿哮喘、40例MP肺炎患儿及15例健康体检儿血NO,LPO,TXB2及CEC水平。结果： 小儿哮喘及MP肺炎急性期血NO,LPO,TXB2,CEC4项指标分别为:哮喘组(162 .27±36.12) μmol/L ,(8.62± 0.87)nmol/ml,(22 9.11± 64.75) pg/ml,(6.13± 1.15)n/0.9μl;MP肺炎组(95.52±33.84)μmol/L ,(5.76± 0 .53)nmol/ml,(388.72±80 .09) pg/ml,(6.36±1.02)n/0 .9μl,分别与对照组 [(68.57±13.80 ) μmol/L ,(4.62± 1.80 )nmol/ml,(105.76±20.10)pg/ml,(4.40±1.04)n/0 .9μl]相比,均增高显著,差异有显著性意义(P<0.01)。其中哮喘组血中NO ,LPO较MP肺炎组增高显著(P<0.01);MP肺炎组TXB2 较哮喘组增高明显 (P<0.01)。恢复期两种疾病所有指标均降低,TXB2,LPO已降至正常范围,而NO ,CEC在两周后[哮喘组(82.64±20.56)μmol/L,(5.41±1.29)n/0.9μl,MP肺炎组 (86.12±21.34)μmol/L,(5.57±1.12 )n/0 .9μl]仍高于正常对照组(P<0.05或0.01)。结论： 本研究提示

Abstract:

OBJECTIVE: To study the roles of nitric oxide (NO) and oxygen free radical (OFR) in the pathogenesis in children with asthma or Mycoplasma (MP) pneumonia. METHODS: The levels of NO, lipid peroxidation (LPO), thromboxane B 2 (TXB 2) and circulating endothelial cells (CEC) were detected in 36 children with asthma (asthma group), 40 MP pneumonia children (MP group) and 15 normal children (control group). RESULTS: The levels of NO, LPO, TXB 2 and CEC in the acute period in the asthma group [( 162.27 ± 36.12 ) μmol/L,( 8.62 ±0.87 ) nmol/ml,( 229.11 ± 64.75 ) pg/ml and ( 6.13 ± 1.15 ) n/ 0.9 μl , respectively] and in the MP group [( 95.52 ± 33.84 ) μmol/L,( 5.76 ± 0.53 ) nmol/ml,( 388.72 ± 80.09 ) pg/ml and ( 6.36 ± 1.02 ) n/ 0.9 μl , respectively] were significantly higher than those in the control group [( 68.57 ± 13.80 ) μmol/L,( 4.62 ± 1.80 ) nmol/ml,( 105.76 ± 20.10 ) pg/ml and ( 4.40 ± 1.04 ) n/ 0.9 μl , respectively](P< 0.01 ). The levels of NO and LPO in the asthma group were much higher thanthose in the MP group (P< 0.01 ), while the TXB 2 level was higher in the MP group than that in the asthma group(P< 0.01 ). The above 4 index levels decreased both in the asthma and MP groups in the recovery period. Of them, the TXB 2 and LPO levels were lowered to normal, while the NO and CEC levels remained higher ( 82.64 ± 20.56 ) μmol/L and ( 5.41 ± 1.29 ) nmol/L in the asthma group; ( 86.12 ± 21.34 ) μmol/L and ( 5.57 ± 1.12 ) n/ 0.9 μl in the MP group] than those in the control group two weeks later (P< 0.05 or 0.01 ). than those in the control group two weeks later (P< 0.05 or 0.01 ). CONCLUSIONS: Both NO and OFR take part in the pathogenesis of asthma and MP pneumonia. NO and LPO appear to be the sensitive indices for asthma and TXB 2 for MP pneumonia. NO could show the extent of tissue repair after injuries.