黄芩甙对H2O2诱导星形胶质细胞NF-κB/IκBα表达的影响

Effect of Baicalin on the Expression of Nuclear Factor κB/IκBα Induced by Hydrogen Peroxide in Astrocytes

摘要:

目的：研究黄芩甙和H2O2对星形胶质细胞中NF-κB/IκBα表达的影响。方法：体外培养星形胶质细胞，分别应用H2O2、黄芩甙及两者联用处理，应用细胞免疫化学法检测NF-κB分子P65亚基核移位情况，Western Blot检测细胞中IκBα表达变化。结果：在星形胶质细胞中，H2O2可使P65由胞浆向胞核移位，并降低IκBα的表达水平；黄芩甙自身不会导致P65核移位及影响IκBα表达水平，但可减少H2O2所致P65亚基胞核移位及细胞内IκBα降低的程度。结论：黄芩甙能减轻H2O2所致星形胶质细胞IκBα异常降低及NF-κB异常活化

Abstract:

OBJECTIVE: To investigate the effect of baicalin, a flavonoid extracted from the radix of  Scutellaria baicalensis Georgi, on the expression of nuclear factor (NF) κB/IκBα induced by hydrogen peroxide (H 2O 2) in astrocytes. METHODS: Astrocytes were cultured in vitro, and treated with various concentrations of baicalin or/and H 2O 2. Immunocytochemistry was used to determine the translocation of P65 from cytoplasm to nucleus; and Western Blot was used to determine the expression change of IκBα in astrocytes. RESULTS: In astrocytes, H 2O 2 could induce the translocation of P65, a subunit of NF κB, from cytoplasm to nucleus, and reduce the level of IκBα expression, while baicalin could inhibit this effect. CONCLUSIONS:Baicalin may decrease the degree of IκBα degradation and NF κB activation induced by H 2O 2 in astrocytes.