幼年大鼠反复热性惊厥脑损伤的研究

Brain Damage Induced by Repeated Febrile Convulsions in Immature Rats

摘要:

目的：观察反复热性惊厥在幼年大鼠引起的脑损伤。方法：65只16d 龄大鼠随机分为正常对照组、高热对照组和热性惊厥组，利用热水浴诱导惊厥模型。采用Morris水迷宫方法检测大鼠的空间学习记忆能力；采用尼氏染色、TUNEL染色和电镜方法观察神经元损伤情况。结果：热性惊厥组大鼠寻找平台潜伏期较正常对照组明显延长［(8.33±9.83) s vs (4.00±2.96) s］，差异有显著性(P<0.01)；尼氏染色未见明显异常；TUNEL染色显示惊厥组大鼠海马区阳性细胞数较正常对照组和高热对照组增加(9.67±5.52 vs 5.08±4.12，9.67±5.52 vs 3.17±3.35)，差异有显著性(P<0.05)；电镜观察惊厥组大鼠可见线粒体肿胀、嵴模糊或断裂、空泡形成等。结论：在未成年大鼠，反复热性惊厥可引起脑损伤

Abstract:

OBJECTIVE: To study the effect of brain damage induced by repeated febrile convulsions (FC) during early life in rats. METHODS: Sixty five 16 d rats were randomly divided into two groups: normal control group (n=15) and warm water treated group (n=50), and the latter was further divided into FC and non FC groups. The rat FC model was developed by warm water immersion (45℃). The memory capacity was tested by using a Morris water maze method. Neuronal changes were detected by thionine stain, TUNEL stain and electron microscopy. RESULTS: The searching plat form latency of rats with FC was significantly longer than that of the normal controls [( 8.33 ± 9.83 ) s vs ( 4.00 ± 2.96 ) s; P< 0.01 ]. The number of TUNEL positive neurons significantly increased in the FC group compared with that of the normal control and non FC groups [( 9.67 ± 5.52 ) vs ( 5.08 ± 4.12 ); ( 9.67 ± 5.52 ) vs ( 3.17 ± 3.35 ), both P< 0.05 ]. The mitochodrion changes, manifesting as swelling, ridge unclear or disappeared and vacuolar appearcance, were found electron microscopically, whereas no significant change was demonstrated by thionine stain. CONCLUSIONS:Repeated FC may induce brain damage in immature rats.