地塞米松对大鼠肾小球系膜细胞增生水平的影响

Effect of Dexamathasone on Glomerulus Mesangial Cell Proliferation in Rats

摘要:

目的：探讨地塞米松(DXM)对大鼠肾小球系膜细胞(GMCs)增生作用的影响。方法：培养大鼠GMCs细胞，分空白对照组、LPS组和DXM组。DXM选用3种浓度，分别为250 ng/孔、1 000 ng/孔和4 000 ng/孔。LPS终浓度为5 mg/孔。采用MTT掺入方法，于 24 h 和48 h观察3组中GMCs增生水平。结果：DXM组在浓度为4 000 ng/孔时48 h的增生水平为(0.251±0.056) ng/L，低于LPS组和GMCs组［(0.787±0.110)，(0.678±0.101) ng/L］，差异有显著性(P<0.05)。DXM浓度为4 000 ng/孔时GMCs增生水平于48 h抑制最为显著。结论：DXM能抑制大鼠GMCs增生。

Abstract:

OBJECTIVE: To study the effect of dexamethasone (DXM) on the proliferation of rat glomerulus mesangial cells (GMCs). METHODS: The GMCs lines of the rat were cultured in virto. Both DXM and lipopolysaccharide (LPS) or LPS alone were added into the culture medium (DXM group or LPS group); in the others, neither DXM nor LPS was added and they were used as controls. The GMCs proliferative level was detected by the MTT method at 24 and 48 h of the experiment. RESULTS: The GMCs proliferative level in the DXM group of 4 000 ng/well at 48 h [( 0.251 ± 0.056 ) ng/L] was lower than that of the LPS group [( 0.787 ± 0.110 ) ng/L] and the controls [( 0.678 ± 0.101 ) ng/L](P< 0.05 ); and the GMCs proliferative inhibition reached a peak at 48 h. CONCLUSIONS: DXM may inhibit the proliferation of GMCs in rats.