黄芪注射液对人巨细胞病毒感染的粒-单系祖细胞体外增殖的影响

Effect of Astragalus on the Proliferation of Colony Forming Units Granulocute/Monocyte infected by Human Cytomegalovirus in Vitro

摘要:

目的：探讨黄芪注射液对人巨细胞病毒(HCMV)感染的粒—单系祖细胞(CFU-GM)体外增殖的影响。方法：采用造血祖细胞体外培养技术，用黄芪干预HCMV感染的CFU-GM，观察计数CFU-GM的产率，大小，峰期及维持时间。结果：①黄芪组每2×105个细胞中CFU-GM簇和集落的产率分别为 333.33±12.69 和81.23±4.93，病毒组每2×105个细胞中簇和集落的产率分别为 167.80±16.63，53.70±1.67，两者相比差异有显著性(P<0.01)。②黄芪组大集落的比例占(9.5±0.8)%，明显高于病毒组(2.7±1.0)%，P<0.01。③黄芪组大集落峰期出现早，集落和大集落维持时间长。结论：在体外黄芪注射液可以促进HCMV感染的CFU-GM增殖。

Abstract:

OBJECTIVE: To study the effect of astragalus on the proliferative ability of colony forming units granulocyte/monocyte (CFU GM) infected by human cytomegalovirus (HCMV) in vitro. METHODS: With the culture technique of CFU GM in vitro, the proliferation of CFU GM infected by HCMV was assayed in a group treated with astragalus and another one without astragalus. RESULTS: ①The numbers of CFU GM clusters and colonies in 2×10 5 cells in the astragalus treated group [( 333.33 ± 12.69 ) and ( 81.23 ± 4.93 ), respectively] were significantly greater than those in the un treated group [( 167.80 ± 16.63 ) and ( 53.70 ± 1.67 ), respectively](P< 0.01 ). ②The proportion of the production of big colonies in the astragalus treated group [( 9.5 ± 0.8 )%] was significantly higher than that in the un treated group [( 2.7 ± 1.0 )%] (P< 0.01 ). ③The peak time of big colonies appeared earlier, and the colony and big colony could last longer in the astagalus treated group compared with the un treated group. CONCLUSIONS: Astagalus may promote the proliferation of CFU GM infected by HCMV.