NF-κB在黄芩甙诱导大鼠骨髓基质细胞分化为神经细胞中的作用(英文)

Role of NF-κB in Baicalin Induced Differentiation of Rat Bone Marrow Stromal Cells into Neurons

摘要:

目的：研究核因子-κB(NF-κB)在中药单体黄芩甙体外诱导骨髓基质细胞分化为神经细胞的作用。方法：实验分为黄芩甙组和对照组。黄芩甙组采用黄芩甙作为主要诱导剂,诱导成年大鼠骨髓基质细胞6d。对照组仅用无血清培养基诱导。采用间接免疫荧光染色检测神经细胞标记蛋白和NF-κB亚基P65的表达。结果：黄芩甙诱导6d,骨髓基质细胞形成较典型的神经细胞形态,同时表达多种神经细胞标记蛋白;对照组未见神经细胞标记蛋白表达。而且,对照组诱导6d,P65信号出现核移位,胞浆中信号明显减弱到(18.4±3.0)％,黄芩甙组P65信号(84.8±3.0)％主要仍在胞浆。结论：黄芩甙可以抑制NF-κB的活化,在骨髓基质细胞诱导分化为神经细胞过程中可能起到一定的作用。 [中国当代儿科杂志,2003,5(1):1—4]

Abstract:

OBJECTIVE: To explore whether the transcription factor NF-κB takes part in baicalin-induced differentiation of bone marrow stromal cells (MSCs) into neurons. METHODS: MSCs from adult rats were induced by baicalin in the serum-free medium for 6 hs. The un-treated cells, as the control group, were only induced in the serum-free medium without baicalin. The expression of neuronal specific markers was evaluated by indirect immunofluorescence cytochemistry staining. The activity of NF-κB was measured by the presence of the NF-κB subunit RelA ( p65) translocated into the nucleus in the same way. RESULTS: After the induction by baicalin, MSCs displayed neuronal morphologies, such as pyramidal cell bodies and extensive networks of processes, and the expression of neuron-specific markers was detectable 6 ds after the induction. Neuronal specific marker proteins did not express in the control group. Six days after the induction, the P65 positive rate in the cytoplasm of the control group decreased to (18.4 ± 3.0) % , while in the baicalin group, the P65 positive rate in the cytoplasm was (84.8 ± 3.0)%. CONCLUSIONS: Baicalin may inhibit the activation of NF-κB, which may act in the differentiation of MSCs into neurons. [Chin J Contemp Pediatr, 2003, 5(1): 1 - 4]