我国再障患儿血清中人细小病毒B19 NS基因序列测定及变异分析(英文)

Sequence Analysis and Genetic Variation of NS Gene of Human Parvovirus B19 Isolated from Chinese Children with Acute Aplastic Anemia

摘要:

目的：探讨B19病毒中国株NS基因的变异。方法：采用聚合酶链反应(PCR)技术,从中国再障患儿血清中扩增人细小病毒B19-XA17,XA18,XA20株的NS基因片段,进行核苷酸序列分析。结果：人细小病毒B19-XA17、XA18、XA20株的NS基因核苷酸序列被测定,测序结果与国外Au株基因核昔的序列比较,有4个核苷酸突变,并引起编码的4个氨基酸发生改变。结论：我国B19病毒NS基因序列与Au株相比有变异。

Abstract:

OBJECTIVE:To explore the NS gene structure and mutation of Chinese strains of human Parvovirus B19 (HPV B19). METHODS: NS genes of R19-XA17, XA18 and XA20 strains from three Chinese children with acute aplastic anemia were amplified by PCR, and then the amplified products were sequenced directly. RESULTS: Compared with the sequence of Au strain, four bases of NS genes of B19-XA17, XA18 and XA20 strains changed and their coding amino acids changed also. CONCLUSIONS: Genetic diversity exists in mucleotide sequences of NS gene of HPV B19 isolated from the serum of Chinese children. [Chin J Contemp Pediatr, 2003, 5(1): 5 - 7]