NO对慢性病贫血大鼠转铁蛋白受体表达的影响(英文)

Effect of Nitric Oxide on the Expression of Transferrin Receptor in Rats with Anemia in Chronic Disease

摘要:

目的：探讨一氧化氮(NO)在慢性病贫血(ACD)发病中的作用及对骨髓血细胞转铁蛋白受体(TfR)的影响,为ACD的防治提供实验依据。方法：用福氏完全佐剂建立传统的类风湿性关节炎大鼠动物模型,在此基础上通过反复注射福氏完全佐剂,建立ACD大鼠动物模型。利用此模型观察对照组、炎症组及炎症十一氧化氮合酶(NOS)抑制剂组的NO浓度的改变、贫血的程度及与TfR的关系。结果：炎症组NO、NOS浓度显著高于对照组,贫血明显,TfR表达强度低于对照组,差异有显著性(P<0.01);用NOS抑制剂后,NO和NOS水平低于炎症组但仍高于对照组,贫血改善,TfR介于炎症组与对照组之间,差异有显著性(P<0.01)。结论：NO参与了ACD的发病及ACD时TfR的调节,为从NO对TfR影响角度进一步认识ACD的发病机制提供了实验依据;及早降低NO水平,有利于阻止贫血的发展,为ACD的治疗提供一条新途径。

Abstract:

OBJECTIVE: To study the influence of nitric oxide (NO) in rats with anemia in chronic disease (ACD) and the effect of NO on the expression of transferrin receptor (TfR) in bone marrows and to provide experimental evidence for the prevention and treatment of ACD. METHODS: The conventional animal model of rheumatoid arthritic (RA) was established by injection of Freund's complete adjuvant. On the basis of this model, we injected Freund's complete adjuvant repeatedly to establish the ACD model. The rats were randomly assigned into three groups (Group A: control group; Group B: inflammatory group; Group C: inflammatory + NO inhibitory agent group). The histopathological changes of the toe joints of the rats were observed and the contents of NO, Hb and nitric oxide synthetase (NOS), and the expression of TfR were measured in the three groups. RESULTS: In Group B, the contents of NO and NOS in the serum were higher than those in Group A; TfR expression in bone marrow cells was lower than that in Group A, and anemia was more severe than that in Group A. After administrating NOS inhibitory agent (L-NAME), anemia was improved; the contents of NO and NOS remarkably decreased compared with those in Group B, but were still higher than those in Group A; TfR in bone marrow cells obviously increased compared with that in Group B, but was still lower than that in Group A. CONCLUSIONS: NO may play an important role in the pathogenesis of ACD and regulation of TfR on ACD, thereby providing experimental evidence for further study of the pathogenesis of ACD. It is helpful in hindering the development of anemia by reducing the NO level as early as possible, and is a new way of treating ACD.