姜黄素诱导HSP70及对大鼠感染性脑水肿保护作用的研究

Heat shock protein-70 expression induced by curcumin and the protective effect of curcumin on infections brain edema in rats

摘要:

目的：研究姜黄素预处理诱导HSP 70表达的量效和时效关系及对感染性脑水肿的保护作用。方法：①量效及时效关系的研究:SD大鼠随机分为空白对照组、二甲亚砜(DMSO)组、热休克(HS)组和姜黄素(CUR)组;再根据姜黄素的剂量(mg/kg)分为80 mg,40 mg,20 mg及10 mg组。根据观察时间分为0,2,4,6,12,16,24 h及48 h组。Western印迹分析检测脑组织HSP70。②脑水肿保护作用研究:SD大鼠随机分为生理盐水对照组(NS)、感染性脑水肿组(PB)、二甲亚砜组、热休克预处理组和姜黄素预处理组。制备感染性脑水肿模型,用干湿法测定脑含水量;原子光谱吸收法测定脑组织钠、钾含量。结果：正常情况下,脑组织有一定量HSP70的表达,DMSO,CUR 20 mg和CUR 10 mg组HSP70表达无明显增多;HS,CUR 40 mg及CUR 80 mg组HSP70明显增加,以CUR 40 mg组明显,差异有显著性(P<0.01)。HSP70在注射姜黄素后2 h开始表达,16 h达高峰,24～48 h处于稳定状态。HS组、CUR组的脑含水量和脑组织钠离子含量与PB组比较有明显降低,差异有显著性(P<0.01)。结论：姜黄素可诱导大鼠脑组织HSP70的产生,有剂量及时间依赖关系。姜黄素预处理对大鼠感染性脑水肿有保护作用。

Abstract:

OBJECTIVE: To study the dose-effect and time-effect of heat shock protein-70 (HSP70) expression induced by curcumin, an antioxidant compound extracted from the spice tumeric, and the protective effect of curcumin on infectious brain edema in rats. METHODS: Dose-effect and time-effect: 24 SD rats were randomly assigned into four groups: control group, dimethyl sulfaxide (DMSO) group, heat shock group and curcumin group. The rats in the curcumin group were subdivided into groups receiving 80, 40, 20 and 10 mg of curcumin, respectively. Another 24 SD rats injected with 40 mg curcumin were divided into groups sacrificed at 0 h, 2 h, 4 h, 6 h, 12 h, 16 h, 24 h, and 48 h. HSP70 expression was detected by the Western blotting analysis. Protective effect of curcumin on infectious brain edema: 52 SD rats were assigned into five groups: normal control group, infectious brain edema group, DMSO pretreatmentgroup (DMSO group), heat shock pretreatment group (HS group), and curcumin pretreatment group (CUR group). Water content and Na+ and K+ contents in brain tissues were measured. RESULTS: Compared with the control group, HSP70 expressions of the heat shock group, 40 mg curcumin group and 80 mg curcumin group significantly increased, especially in the 40 mg group ( P <0.01). HSP70 expression gradually increased with the time after the injection of curcumin, peaking at 16 h and reaching a plateau at 24 h and 48 h. The contents of water and Na+ in brain tissues significantly decreased in the HS and CUR groups compared with the un-treated infectious brain edema group. CONCLUSIONS: HSP70 expression can be induced by pretreatment with curcumin, and there are dose-effects and time-effects. The protective effect of curcumin against infectious brain edema may be a consequence of increased HSP70 expression in rats.