母婴输血传播病毒感染及垂直传播的分子流行病学研究

Molecular Epidemiology Study on Mother to Infant Transmission of Transfusion Transmitted Virus

摘要:

目的：探讨孕产妇输血传播病毒(TTV)感染情况及其母婴传播途径。方法：采用巢式聚合酶链反应(n PCR)技术对广州市4 90例孕产妇静脉血及其新生儿脐血标本进行TTVDNA扩增,并对 8例孕产妇静脉血及新生儿脐血TTVDNA均阳性的PCR产物进行克隆和测序。结果：87例孕产妇静脉血及12例新生儿脐血检测出TTVDNA ,孕产妇TTVDNA阳性率为17.8% ,母婴垂直传播率为13.8%。TTV广州分离株核苷酸序列与日本株的同源性为85 .3%～98.2 %。结论：孕产妇TTV感染率较高,TTV可经胎盘传递给胎儿而引起感染

Abstract:

OBJECTIVE: To study the incidence of transfusion transmitted virus (TTV) infection in pregnant women and the route of TTV infection. METHODS: TTV DNA was detected by nested polymerase chain reaction (n PCR) technique in serum of 490 pregnant women and in umbilical blood of their infants in Guangzhou City. PCR products from the 8 women and their infants with positive TTV DNA were cloned and sequenced. RESULTS: The positive TTV DNA occurred in 87 mothers ( 17.8% ) and 12 babies born to them, with 13.8% of incidence of mother to infant transmission. The homology of nucleotide sequence of TTV isolates from Guangzhou of China and from Japan was 85.3% - 98.2% . CONCLUSIONS: There is a high incidence of TTV infection in pregant women. TTV can be transmitted via placenta (vertical transmission).