柴胡 黄芩 炙甘草对小鼠CVB3心肌炎治疗作用的研究

Therapeutic Effects of Bupleurum Root, Scutellaria Root and Baked Licorice on Viral Myocarditis Caused by Coxsackievirus B3 in Mice

摘要:

目的：探讨单剂柴胡、黄芩、炙甘草对柯萨奇病毒B3(CVB3)感染的病毒性心肌炎小鼠的治疗作用。方法：用CVB3感染Balb/C小鼠造成病毒性心肌炎模型,分别用生理盐水、柴胡、黄芩、炙甘草灌胃治疗,观察病毒感染后不同时间点的心肌组织病理变化。结果：柴胡组小鼠在感染后第 5 ,7,10天心肌病理积分为1.33±0 .82 ,1.6 0± 0 .6 3和 1.87± 0 .74 ,明显低于对照组 (2 .2 2± 1.37,3.2 7± 0 .96 ,2 .6 0± 1.0 6 ) (P <0 .0 5或 0 .0 1)。炙甘草组小鼠在感染后第5 ,7天心肌病理积分为 1.2 6± 0 .88和 1.80± 0 .6 8,明显低于相应对照组 (P <0 .0 5或0 .0 1)。黄芩组小鼠在感染第14 ,2 1天后心肌病理积分(1.5 3± 0 .74 ,0 .80± 0 .5 6 )与对照组 (2 .33± 0 .98,1.4 0±0 .83)比较差异有显著性 (P <0 .0 5 )。结论：单剂柴胡、黄芩、炙甘草在不同的时间点对心肌细胞起保护作用

Abstract:

OBJECTIVE: To study the effects of bupleurum root, scutellaria root and baked licorice respectively, on viral myocarditis caused by coxsackievirus B3 (CVB3) in mice. METHODS: The viral myocarditis model was established by injecting CVB3 in 100 Balb/C mice. Normal saline (served as the control group), bupleurum root, scutellaria root and baked licorice were administrated by intragastric route in 25 mice respectively, with the dosage of 0.5 ml , once daily for 2 weeks. The pathologic changes in myocardium of mice were evaluated with the routine histopathological method. RESULTS: The pathology scores of myocardium on the 5th, 7th and 10th days after treatment in the bupleurum root group ( 1.33 ± 0.82 , 1.60 ± 0.63 and 1.87 ± 0.74 , respectively) were significantly lower than those of the controls ( 2.22 ± 1.37 , 3.27 ± 0.96 and 2.60 ± 1.06 , respectively) (P< 0.05 or 0.01 ). On the 5th and 7th days after the treatment with baked licorice, there were significant differences in the pathology scores of mycocardium between the baked licorice group (1.26±0.88 and 1.80±0.68) and the control group (P< 0.05 or 0.01 ). Scutellaria root took effects later. The pathology scores of myocardium in the scutellaria root group significantly decreased on day 14 and day 21 after treatment compared with those of the control group ( 1.53 ± 0.74 vs 2.33 ± 0.98 and 0.80 ± 0.56 vs 1.40 ± 0.83 , respectively; P< 0.05 ). CONCLUSIONS: Bupleurum root, scutellaria and baked licorice may have protective effects on the myocardium at different time after medication.