窒息新生儿血浆非蛋白结合铁的测定及意义

Determination of Plasma Nonprotein Bound Iron in Newborns with Asphyxia

摘要:

目的：探讨非蛋白结合铁在新生儿窒息后再灌注损伤中的作用。方法：用克服基体效应的Bleomycin方法，分别测定20例窒息新生儿生后6h内、6～12h和12～72h的血浆非蛋白结合铁(NPBI)，并取20例正常新生儿于出生6h内测定其血浆非蛋白结合铁作为对照。结果：窒息组NPBI阳性率明显高于 对照组(80% vs 20%)，P<0.01。窒息组血浆非蛋白结合铁水平在生后6h内为［(4.14±2.41) μmol/L］、6～12 h为(2.26±2.21) μmol/L，均比对照组［(0.28±0.79)μmol/L］显著升高(P＜0.05＝，尤以生后6 h内为最高。结论：非蛋白结合铁可能在新生儿窒息后再灌注损伤中起着重要的作用。

Abstract:

OBJECTIVE: To study the effect of nonprotein bound iron (NPBI) on neonatal postasphyxial reperfusion injury. METHODS: Plasma concentrations of NPBI from 20 asphyxiated newborns were measured serially with the matrix effect free bleomycin assay during 0 to 6 hours, 6 to 12 hours and 12 to 72 hours after birth. They were then compared with those from 20 healthy newborns within 6 hours after birth (controls). RESULTS: The positive NPBI rate was significantly higher in asphyxiated newborns than that in the controls (80% vs 20%). NPBI was significantly elevated in asphyxiated newborns during 0 to 6 hours [( 4.14 ± 2.41 ) μmol/L] and 6 to 12 hours [( 2.26 ± 2.21 ) μmol/L] compared with that in the controls [( 0.28 ± 0.79 ) μmol/L] (P< 0.05 ). The highest level of NPBI was noted during 0 to 6 hours after birth. CONCLUSIONS: NPBI may play an important role in neonatal postasphyxial reperfusion injury.