急性脑缺血小鼠脑组织中转化生长因子-β1的表达及意义

Expression of Transforming Growth Factor β in Brain Tissue After Acute Cerebral Ischemia in Mice

摘要:

目的：转化生长因子-β1(TGF-β1)在脑损伤的发生、发展等过程中发挥着重要作用,实验性脑损伤动物可有TGF-β1的高表达。本文探讨急性脑缺血小鼠脑组织中TGF-β1的表达情况及其意义。方法：20只小鼠随机分为正常对照组和脑缺血模型组。通过结扎小鼠左侧颈总动脉制备脑缺血模型。原位杂交方法检测脑组织中TGF-β1 mRNA的表达。结果：模型组小鼠脑组织中TGF-β1的表达程度较高,其中8例为Ⅲ级,2例为Ⅱ级;而在正常对照组小鼠脑组织中基本不表达TGF-β1,仅1例表达为Ⅰ级。结论：TGF-β1可能与动物缺血性脑损伤的发生有关。

Abstract:

OBJECTIVE: In recent years there has been an increasing interest in the evaluation of the neuronal responses to ischemic insult. Some cytokines, including transforming growth factor-beta 1 (TGF-β1) , are overexpressed after experimental brain demage in animals. This study aimed at the expression of transforming growth factor-betal (TGF-β1) in brain tissue after acute cerebral ischemia in mice. METHODS: Twenty mice were randomly assigned into a normal control group and a cerebral ischemia model group (n = 10 for each). The models of cerebral ischemia were established through ligating the left common carotid artery. The level of TGF-β1 mRNA expression was detected by in situ hybridization. RESULTS: In the model group, the brain tissue presented high levels of TGF-β1 mRNA expression: Grade Ⅲ in 8 cases and Grade Ⅱ in 2 cases, whereas in the normal control group only one case presented Grade Ⅰof TGF-β1 mRNA expression. CONCLUSIONS: TGF-β1 may participate in the pathogenesis of acute cerebral ischemia in mice.