黄芪和大青叶治疗小鼠病毒性心肌炎的对比研究

Control Study of Astragalus Membranaceus and Folium Isatidis in Treatment of Mur ine Viral Myocarditis

摘要:

目的：通过中药单剂黄芪、大青叶治疗小鼠病毒性心肌炎(VM C)的疗效观察，探讨VMC的有效治疗措施。方法：Balb/C小鼠给予柯萨奇病 毒B3(CVB3)稀释液腹腔注射后，随机分为感染对照组、黄芪治疗组和大青叶治疗组，于CVB3 感染后第3，5，7，10，14，21天分批处死，作HE染色，比较各组小鼠心肌病变积分，并行 心肌组织病毒分离。于感染后第7天各组小鼠电镜观察心肌细胞超微结构改变。结果：黄芪治疗组自CVB3感染后第5～21天、大青叶治疗组在CVB3感染后第5天和7天时，其心肌病变积分较感染对照组明显减轻(P＜0.05)。而大青叶治疗组CVB3感染后 第10～21天心肌病变积分与感染对照组比较差异无显著性(P＞0.05)。电镜观察发 现感染对照组小鼠心肌细胞线粒体肿胀、嵴断裂、肌浆网扩张、肌丝溶解，治疗组上述病变轻，且大青叶治疗组可见大量溶酶体。病毒分离结果显示3组小鼠CVB3感染后第3天即可分离 出病毒，阳性率均为66.7%，第5～10天3组均为100%，第14天感染组病毒分离阳性率为33.3%，治疗组均未分离到病毒，第21天时3组均未分离出病毒。不同时间点的3组间病 毒分离阳性率差异无显著性(P>0.05)。结论：黄芪具有保护心肌 的作用。大青叶在CVB3感染早期对VMC的病理改变具有显著影响。

Abstract:

OBJECTIVE: To study the curative effect of two Chinese traditional medicines, astragalus membranaceus and folium isatidis, on murine viral myocarditis (VMC). METHODS: Balb/C mice were injected intraperiloneally with a diluted solution of coxsackievirus B3 (CVB3), and then randomly divided into three groups; infected control group, astragalus membranaceus treated group and folium isatidis treated group. The mice were sacrificed on days 3, 5, 7, 10, 14 and 21 after injection. The myocardial pathological score was estimated and viruses from myocardium were separated. In addition, the myocardial ultrastructure was detected under electon microscope on day 7 of post-injection. RESULTS: Compared with the myocardial pathological score of the infected control group, thxxse of both the astragalus membranaceus treated group (from day 5 to day 21) and folium isatidis treated group (on day 5 and day 7 post-injection) were significantly decreased ( P <0.05). There was no difference in the myocardial score after 10 days of infection between the folium isatidis group and the infected control group. The ultrathin section of myocardium in the infected control group revealed mitochondria! swelling, mitochondrial crista rupture and sarcoplasmic reliculum dilatation under electron-microscope. The pathological changes were relieved after treatment in both the astrgalus membranaceus and folium isatidis groups. Moreover, there were a lot of lysosomes in myocardium of the folium isatidis treated group. The results of viral isolation were positive on day 3 of post-infection in the three groups, with the same positive rate of 66. 7% . The same positive rate of viral isolation (100% ) was also found from day 5 to day 10 of post-infection in the three groups. On day 14 of post-infection, the positive viral isolation was noted only in the infected control group (33.3%) but failed in all three groups on day 21 of post-infection. CONCLUSIONS: Astragalus membranaceus may have a protective effect on myocardial cells. Folium isatidis could relieve myocardial lesions in the early stage of CVB3-induced myocarditis.