参麦注射液 生长激素 硫酸锌 维生素C对心肌细胞存活力的保护作用

Protective Effects of Shenmai Injection, Growth Hormone, Zinc Sulfate and Vitamin C on Cardiac Myocytes Viability

摘要:

目的：氧自由基和血管紧张素Ⅱ(AngⅡ)过多是造成心肌损害的重要机制,为有效保护心肌,为心肌损害治疗开辟一个新途径,本文研究参麦注射液(SMI)、生长激素(GH)、硫酸锌(ZnSO4)、维生素C(VitC)对过氧化氢(H2O2)或AngⅡ损伤下降的心肌细胞存活力(CMV)的影响。方法：用新生大鼠心肌细胞加入H2O2(200 μmol/L,500μmol/L)或AngⅡ(10-6mol/L,10-7mol/L)使CMV下降作为对照组(共4组);在加入H2O2或AngⅡ的同时加入SMI(5 ml/L和10 ml/L),VitC(50 mg/L),ZnSO4(15μmol/L和60 μmol/L)或GH(250 μmol/L和500μmol/L)作为保护组。检测各组在加药24 h后的CMV增高率。结果：SMI,GH,ZnSO4和VitC均可显著提高因H2O2损伤而下降的CMV增高率。以SMI 10 ml/L的效果最好;其次为ZnSO4 60 μmol/L;再其次为SMI 5 ml/L,VitC 50 mg/L;最差为GH 500 μmol/L,GH 250 μmol/L和ZnSO4 15 μmol/L,其中SMI 5 ml/L和VitC 50 mg/L间差异无显著性,后3者间差异也无显著性。SMI和GH可显著提高AngⅡ所致的CMV下降,以SMI 10 ml/L效果最好;其余依次为SMI 5 ml/L,GH 500 μmol/L和GH 250μmol/L,但后2者间差异无显著性(P>0.05)。结论：SMI和GH对因H2O2或AngⅡ损伤下降的CMV有显著提高作用。

Abstract:

OBJECTIVE: The increase of oxygen-derived free radicals (OFR) or angiotensin Ⅱ(Ang Ⅱ ) in blood is one of the important causative agents of myocardium lesions. To develop a remedy for treatment of myocardium lesions induced by OFR or Ang Ⅱ , this study aims at exploring the protective effects of shenmai injection (SMI), growth hormone (GH), zinc sulfate (ZnSO4) and vitamin C (Vit C) on the cardiac myocytes injured by hydrogen peroxide (H2O2) or Ang Ⅱ. METHODS: H2O2 with the concentrations of 250 and 500 μmol/L or Ang Ⅱ with the concentrations of 10 6 and 10 7 mol/L were added respectively to the cardiac myocytes of neonatal rats so as to reduce the cardiac myocytes viability ( CMV). The injured cardica myocytes were administrated respectively with 5 ml/L and 10 ml/L (concentration) of SMI, 50 mg/L of Vit C, 15μmol/L and 60 μmol/L of ZnSO4, and 250 μmol/L and 500 μmol/L of GH. The increasing rate of CMV was measured at 24 h after medication. RESULTS: SMI, GH, ZnSO4 and VitC could all significantly elevate the increasing rate of CMV which had been decreased by H2O2 injury. The best effect was made by the SMI 10 ml/L group, followed in turn the ZnSO4 60 μmol/L, SMI 5 ml/L, Vit C 50 mg/L, GH 500 μmol/L, 15 μmol/L ZnSO4 and 250 μmol/L GH groups. There was no significant difference between the SMI 5 ml/L group and VitC 50 mg/L group. Nor was there among the GH 500 μmol/L, GH 250 μmol/L and ZnSO4 15 μmol/L groups. The CMV which had been decreased by Ang Ⅱ injury was significantly increased after administrating SMI and GH. The best effect was made by the SMI 10 ml/L group, followed in turn the SMI 5 ml/L, GH 500 μmol/L and GH 250 μmol/L groups. No difference was found among the last two groups. CONCLUSIONS: SMI, GH, ZnSO4 and VitC could all increase CMV which had been decreased by H2O2 injury, and both SMI and GH could increase CMV which had been decreased by Ang Ⅱ injury.