Survivin Caspase-3 mRNA在肾母细胞瘤中的表达及意义

Expression of Survivin and Caspase-3 mRNA in Wilms tumor

摘要:

目的：在大多数肿瘤组织中都发现了Survivin异常高表达现象，它直接抑制Caspase-3，这说明Survivin在肿瘤发生中具有重要作用。本文的目的在于探讨Survivin、Caspase-3表达与肾母细胞瘤发生发展的关系。方法：应用RT-PCR技术检测Survivin、Caspase-3 mRNA在48例肾母细胞瘤、24例癌旁组织标本中的表达。结果：48例肾母细胞瘤组织中Survivin阳性表达率为 72.9%(35/48)，在24例肾母细胞瘤癌旁组织中未检测到Survivin的表达，肾母细胞瘤组织与癌旁组织之间Survivin表达阳性率差异有非常显著性(P<0.01)；48例肾母细胞瘤组织中Caspase-3阳性表达率为 8.3%(4/48)，在24例肾母细胞瘤癌旁组织中Caspase-3阳性表达率为 45.8%(11/24)，肾母细胞瘤组织与癌旁组织之间Caspase-3表达阳性率差异有显著性(P<0.05)。结论：Survivin在肾母细胞瘤组织中高度表达和Caspase-3在肾母细胞瘤中低表达可能与肾母细胞瘤的发生发展有关，与预后的关系有待进一步的探讨。［中国当代儿科杂志，2004, 6(5): 381-384]

Abstract:

OBJECTIVE: Abnormal expression of Survivin has been found in the majority of tumor tissues and it can directly inhibit the expression of Caspase-3. It is suggested that Survivin plays an important role in the development of tumors. This study aims to investigate the expression of Survivin and Caspase-3 mRNA in Wilms tumor. METHODS: Survivin and Caspase-3 mRNA were detected by RT-PCR in 48 specimens from Wilms tumor tissues and 24 from adjacent normal kidney tissues. RESULTS: Survivin mRNA was expressed in 72.9% of Wilms tumor tissue samples, while no Survivin mRNA expression was detected in adjacent normal kidney tissues. In contrast, Caspase-3 mRNA was expressed in only 8.3% of Wilms tumor tissues but 45.8% of adjacent normal kidney samples (P< 0.05). CONCLUSIONS: Increased expressions of Survivin mRNA and decreased expression of Caspase-3 mRNA were found in Wilms tumor, and may be related to the development of Wilms tumor.