儿童血浆促酰化蛋白的测定及意义

Determination of plasma acylation stimulating protein in children

摘要:

目的：介绍一种简易、快速、特异性高的人血浆促酰化蛋白(acylationstimulatingprotein,ASP)的检测技术,并阐明ASP测定意义。方法：161例研究对象,其中健康儿童98例,单纯性肥胖症患儿63例,年龄2～6岁,ELISA方法测定血浆ASP的浓度,波长490nm。结果：该方法最佳线性范围:ASP浓度0.5～10ng/mL,平均批内和批间变异系数(CV)分别为8.17%和9.72%。正常学龄前儿童血浆ASP浓度为69.14±25.58nM;单纯性肥胖症儿童血浆ASP浓度为95.64±36.24nM,明显高于健康儿童(P<0.001);ASP与BMI呈正相关。结论：该检测方法能简易、快速对血浆ASP进行准确定量,在ASP基础和临床相关疾病的研究中有重要的意义。

Abstract:

OBJECTIVE:  To introduce a simple, rapid and stable method for the determination of plasma acylation stimulating protein (ASP) and to explore the value of plasma ASP determination. METHODS: Sixty-three simple obese children and 98 healthy controls were included in this study. The ELISA technique was employed to determine the plasma ASP concentration. RESULTS: The best linearity of test was between 0.5-10 ng/mL. The intra-assay coefficient of variation (CV) was 8.17% and inter-assay CV, 9.72%. The average plasma ASP concentration in healthy children was 69.14±25.58 nM and 95.64±36.24 nM in obese children (P<0.001). Plasma ASP was positively correlated with body mass index in both healthy and obese children (r=0.2883 and 0.337, respectively, both P<0.01). CONCLUSIONS: Plasma ASP can be quantified by the ELISA method. Plasma ASP determination may be recommended as a new method for lipid metabolism determination in children.