丹参对幼年大鼠应激性溃疡预防作用的实验研究

Prophylactic effect of radix salvia miltiorrhiza on gastric stress ulcer in young rats

摘要:

目的：应激性溃疡作为重症患儿病情加重或恶化的标志之一,应引起临床高度重视。儿科药物剂量因其特点与成人不尽相同,不宜直接套用成人防治应激性溃疡的方案,且有关用药的利弊、与医源性肺炎的关系及夜间酸突破现象的治疗等都存在很多分歧,有待于进一步探讨和研究。该试验旨在观察复方丹参对幼年大鼠应激性溃疡(SU)的黏膜损伤有无预防作用。方法：将30只幼年大鼠随机分成5组,即对照组、实验组,实验组据应激前腹腔注射用药分为以下4组:生理盐水2 mL(Ⅰ组)、丹参2次组(Ⅱ组)、丹参4次组(Ⅲ组)、丹参6次组(Ⅳ组),采用束缚-水浸法制作应激性溃疡动物模型,对照组与实验各组应激时间均为3 h,观察各组病理形态学变化及检测各组胃黏膜溃疡指数(UI)。结果：对照组、Ⅰ组胃黏膜溃疡指数分别为57.75±5.71,55.63±7.52,两者之间差异无显著性。Ⅱ,Ⅲ,Ⅳ组胃黏膜溃疡指数逐渐降低,Ⅳ组最低,为12.12±3.62,其中Ⅲ和Ⅳ组与对照组相比,差异有非常显著性(P<0.01)。丹参可明显减轻损伤模型中的UI。结论：丹参对应激性溃疡具有预防作用,其机制尚需继续探讨。

Abstract:

OBJECTIVE: Salviae miltiorrhizae (RSM), a Chinese medicine known as Dan-shen with the effect of promoting blood flow,has been used as a treatment for cardiovascular and cerebrovascular diseases. This study aimed to investigate the prophylactic effect of RSM on acute gastric mucosal injury in young rats. METHODS: Gastric stress ulcer were induced by water immersion restraint stress in 30 Sprague-Dawley young rats. The 30 rats were randomly assigned into Control and Experimental groups. The Experimental group was further subdivided into Normal saline and RSM groups according to the different medicines injected before stress. The rats of the RSM group were randomly administered 2 times, 4 times or 6 times by intraperitoneal injection of RSM, 2 mL each time 8 hourly. The Control group received no treatment. The histological changes of gastric mucosa and gastric mucosal ulcer index (UI) were observed. RESULTS: There was no difference in the UI between the Normal saline and Control groups.RSM pretreatment alleviated the gastric mucosal injuries. The rats that received 4 or 6 times RSM injection had significantly lower UI than the Control group. CONCLUSIONS: RSM has protective effects against gastric stress ulcer in young rats.