过敏性紫癜患儿血清白三烯B_4白介素-5的测定及其临床意义

Serum levels of IL-5 and LTB4 in children with Henoch-Schonlein purpura

摘要:

目的：观察过敏性紫癜患儿血清白介素-5(IL-5)和白三烯B4(LTB4)在不同发病阶段的浓度变化,以探讨IL-5和LTB4在过敏性紫癜发病机制中的作用。方法：采集31例过敏性紫癜患儿急性期和恢复早期血清,以及27例健康儿童血清,应用酶联免疫吸附试验检测血清中IL-5,LTB4和C反应蛋白(CRP)含量。结果：过敏性紫癜患儿的急性期血清IL-5,LTB4和CRP含量明显高于恢复早期(P<0.01),恢复早期血清IL-5,LTB4和CRP含量又显著高于正常组儿童(P<0.01)。IL-5和LTB4变化与CRP呈正相关。结论：过敏性紫癜患儿的急性期血清IL-5和LTB含量升高,恢复早期有所下降,提示IL-5和LTB参与了过敏性紫癜的发病过程。

Abstract:

OBJECTIVE: This study investigated the serum levels of interleukin-5 (IL-5), leukotriene B_4 (LTB_4) and C-reactive protein (CRP) in children with Henoch-Schonlein purpura (HSP) at different phases to explore the role of IL-5 , LTB_4 and CRP in the pathogenesis of HSP. METHODS: Serum levels of IL-5, LTB_4 and CRP in 27 normal children and 31 children with HSP at the acute phase and the early recovery phase were detected using ELISA. RESULTS: The serum levels of IL-5, LTB_4 and CRP in children with HSP were 53.8±4.2 pg/mL, 95.3±12.0 pg/mL and 36.10± 11.78 mg/L , respectively at the acute phase. The values were significantly decreased at the early recovery phase (37.8± 3.9 pg/mL , 45.7±10.1 pg/mL, 18.35±6.43 mg/L; P<0.01), but remained higher than those in normal controls (12.7± 3.2 pg/mL , 17.6±5.7 pg/mL, 4.75±2.85 mg/L; P<0.01). The serum levels of IL-5 and LTB_4 positively correlated to the CRP level. CONCLUSIONS: The serum levels of IL-5 and LTB_4 in children with HSP increased during the acute phase and decreased at the early recovery phase, suggesting that IL-5 and LTB_4 may be involved in the pathogenesis of HSP.