天花粉蛋白对小鼠单纯疱疹病毒感染性脑损伤的保护作用

Protective effects of trichosanthin in Herpes simplex virus-1 encephalitis in mice

摘要:

目的：探讨天花粉蛋白对小鼠实验性单纯疱疹病毒感染性脑损伤的保护作用。方法：动物随机分为3组,空白对照组(空白组),模型对照组(模型组),模型+天花粉蛋白处理组(治疗组)。小鼠颅内接种单纯疱疹病毒I型(HSV-1)建立病毒性脑炎模型,于接种HSV-1前30min腹腔注射天花粉蛋白注射液,接种病毒后1,12,24,48h、4和7d测定脑组织含水量;感染后7d测定脑组织病毒滴定度,观察脑组织形态变化及动物神经症状评分。结果：天花粉蛋白治疗组于接种HSV-1后48h至7d脑组织含水量明显低于对照组,差异有显著性(P<0.05),治疗组脑组织病毒滴度为1.16±0.45,明显低于模型组的2.89±0.44,差异有显著性;治疗组神经缺陷症状评分也明显低于对照组,病理形态观察治疗组脑组织间质水肿较轻,神经元无浓染、无坏死。结论：天花粉蛋白对小鼠实验性单纯疱疹病毒感染性脑损伤具有保护作用。

Abstract:

OBJECTIVE: Trichosanthin (TCS), a ribosome-inactivating protein extracted from the root tuber of Chinese medicinal herb Trichosanthes kirilowii maximowicz, has various pharmacological properties including abortifacient, anti-tumor and anti-virus. This study aimed to evaluate the effects of TCS on infectious brain injury induced by Herpes simplex virus-1 (HSV-1) in mice. METHODS: Ninety mice were randomly assigned into three groups: Normal control group (n=30) , Model group (n=30) and TCS-treated group (n=30). Viral encephalitis was induced by intracranial inoculation of HSV-1 in the latter two groups. The TCS-treated group was injected with TCS 30 minutes before HSV-1 inoculation. The water content of brain tissue was measured at 1, 12, 24 and 48 hrs, and at 4 and 7 days after HSV-1 inoculation. The viral titer of brain tissue and brain histopathological changes were detected at 7 days after HSV-1 inoculation. The neurological deficient scores were determined daily. RESULTS: The water content of brain tissue in the TCS-treated group between 48 hrs and 7 days after HSV-1 inoculation was significantly lower than that in the Model group (P<0.05), although it was significantly higher than that in the Normal control group (P<0.05). The viral titer of brain tissue in the TCS-treated group was markedly lower than that in the Model group (1.16±0.45 vs 2.89±0.44; P<0.05) 7 days after HSV-1 inoculation. The neurological deficient scores of the TCS-treated group after 24 hrs of HSV-1 inoculation were significantly lower than that in the Model group but were higher than those of the Normal control group. TCS treatment resulted in alleviated pathological changes of brain tissue compared with the Model group 7 days after HSV-1 inoculation. CONCLUSIONS: TCS has protective effects against infectious brain injury induced by HSV-1 in mice.