深圳地区学龄前儿童血清甘露聚糖结合凝集素分析

Serum mannan-binding lectin levels in preschool children from Shenzhen

摘要:

目的：甘露聚糖结合凝集素是机体天然免疫的关键组成成分，该研究探讨学龄前儿童血清甘露聚糖结合凝集素的水平及分布特征，为预防儿童感染提供指导依据。方法：随机留取深圳市三所幼儿园118名3～6岁儿童的血清标本，其中男性62例，女性56例，通过酶联免疫吸附法测定血清甘露聚糖结合凝集素的水平。结果：深圳地区学龄前儿童血清甘露聚糖结合凝集素的浓度为779.07±268.98 ng/mL，其中男性值为783.89±252.30 ng/mL，女性值为773.65±288.29 ng/mL，均值无性别差异（P>0.05）。血清甘露聚糖结合凝集素低于500 ng/mL的儿童有16名，占13.6%，其中50～500 ng/mL有14名，50 ng/mL以下2名。结论：深圳地区学龄前儿童血清甘露聚糖结合凝集素的分布范围为779.07±268.98 ng/mL，可为临床和科研工作者提供参考，对血清甘露聚糖结合凝集素低于正常值下限的儿童可以适当使用抗生素预防感染。

Abstract:

OBJECTIVE:Mannan-binding lectin (MBL) is a C-type serum lectin that plays a central role in the innate immune response. At present there is no a reference range of serum MBL levels for children in China. This study investigated serum MBL levels in preschool children from Shenzhen. METHODS: A total of 118 children (62 boys and 56 girls) at ages of 3-6 years and sampled randomly from three kindergartens of Shenzhen were enrolled in this study. Serum MBL concentrations were determined using enzyme-linked immunosorbent assay. RESULTS: The mean serum MBL concentration in these children was 779.07±268.98 ng/mL. There were no significant differences in the value of serum MBL between boys and girls (783.89±252.30 ng/mL vs 773.65±288.29 ng/mL) (P>0.05). Sixteen children (13.6%) had MBL concentrations less than 500 ng/mL (the low limited value used abroad), including 14 cases with 50-500 ng/mL and 2 cases with less than 50 ng/mL. CONCLUSIONS: This study provides a reference range of serum MBL concentration for preschool children. MBL may be a useful marker for the prevention of infection in children.