新生儿窒息后血清诱导人肾小管细胞粘附中性粒细胞的作用及机制

Role of serum from asphyxiated neonates in the inducement of human renal tubular cell adhesion to neutrophils

摘要:

目的研究新生儿窒息后血清诱导的人肾小管细胞粘附中性粒细胞的作用及机制。方法以HK2细胞株为研究对象, 用含20%的窒息后血清作为诱导因素。实验分空白对照组、窒息组(窒息后1，3，7 d组)和吡咯烷二硫氨基甲酸(PDTC)干预组。分别检测各组细胞悬液中髓过氧化物酶（MPO）活性；检测HK2细胞膜ICAM1和细胞核内NFκB的表达。结果窒息后1 d血清组细胞悬液MPO值显著高于各PDTC干预组、对照组及窒息后3，7 d血清组（P<0.01）。窒息后1 d血清组HK2细胞表面ICAM1表达和HK2细胞核NFκB表达亦显著高于各PDTC干预组、对照组及窒息后3，7 d血清组（P<0.01）。结论 窒息后血清可以诱导肾小管上皮细胞活化进而粘附中性粒细胞，其作用机制可能涉及到窒息后血清活化了NF-κB，从而使肾小管上皮细胞膜表面ICAM-1表达增加，进而粘附中性粒细胞。

Abstract:

ObjectiveTo study the role of serum from asphyxiated neonates in the inducement of human renal proximal tubular epithelial cells (HK2) adhesion to neutrophils and possible mechanisms.MethodsHK2 cells were cultured randomly with 20% serum from neonates (1, 3, and 7 days after asphyxia), pyrrolidine dithiocarbamate (PDTC) or placebo. The activity of myeloperoxidase (MPO), an indicator of adhesion ability of HK2 cells to neutrophils in suspensions, was detected by the biochemistry assay. Intercellular adhesion molecule1 (ICAM1) and nuclear factorκB (NFκB) of HK2 cells were examined with the immunohistochemical staining.ResultsThe expression of MPO in the postasphyxial 1day serum treatment group were significantly higher than that in the PDTC treatment and the control groups as well as the postasphyxial 3 and 7day serum treatment groups (P<0.01). The expression of ICAM1 and NFκB in the postasphyxial 1day serum treatment group was also significantly higher than that in the other groups (P<0.01).ConclusionsSerum from asphyxiated neonates can induce HK2 cell adhesion to neutrophils, possibly through activating NFκB and increasing the synthesis and expression of ICAM1 on the surface of renal tubular epithelial cells.