新生大鼠缺氧性脑损伤新模型

A new hypoxic brain damage model in the neonatal rat

摘要:

目的探讨建立新生鼠缺氧性脑损伤新模型，验证新模型的可靠性及优越性。方法7日龄Wistar大鼠随机分为无氧组、RiceVannucci法组、单纯缺血组和正常组。无氧组于0%氧浓度环境中持续7min；RiceVannucci法组采用传统的RiceVannucci法处理；单纯缺血组只结扎左颈总动脉，不缺氧；正常组不作处理。各组处理后测定脑含水量、神经细胞凋亡、神经功能，取脑行苏木精伊红染色，观察组织病理改变。结果无氧组大鼠左脑的含水量［（88.96±0.29）%］和神经细胞凋亡计数［（31.52±5.45）%］明显高于正常组和单纯缺血组（P＜0.01）；与RiceVannucci法组相比，差异无统计学意义，无氧组大鼠右脑含水量［（88.68±0.24）%］和神经细胞凋亡计数［（30.85±5.38）%］明显高于RiceVannucci法组、单纯缺血组和正常组（P＜0.01）；苏木精伊红染色无氧组左右大脑均有病变，RiceVannucci法组仅左脑有病变，单纯缺血组和正常组左右大脑均无病变；与单纯缺血组和正常组相比，无氧组和RiceVannucci法组神经功能明显受损。 结论 置新生大鼠于0%氧浓度环境中持续7 min，成功建立了简便可靠的新生鼠缺氧性脑损伤模型。

Abstract:

ObjectiveThis study aimed to prepare a hypoxic brain damage model in the neonatal rat using a new approach, 0% oxygen exposure, and to explore the reliability and advantages of the new model.MethodsSevendayold Wistar rats were randomly exposed to either 7 minutes of 0% oxygen, to the conventional RiceVannucci method (ischemia + 2 hrs hypoxia exposure), or to left common carotid artery ligation (ischemia). Rat pups which were not subjected to any hypoxiaischemia treatment were used as the control group. Brain water content and neuronal apoptosis were measured. Neurofunctional assessment was performed. Brain pathological changes were observed using hematoxylin and eosin staining.ResultsThe water content (88.96±0.29%) and apoptosis of neurons (31.52±5.45%) of the left brain in the 0% oxygen group were significantly higher than those of the ischemic and the control groups (P<0.01), and similar to those in the RiceVannucci group. The water content（88.68±0.24%） and apoptosis of neurons（30.85±5.38%）of the right brain in the 0% oxygen group were significantly higher than those of the RiceVannucci, the ischemic and the control groups（P<0.01）. Both side brains of the 0% oxygen group showed pathological injuries, but only left brain of the RiceVannucci group had pathological changes. No pathological abnormalities were seen in the ischemic and the control groups. Significant neurofunctional impairments were found in the 0% oxygen and the RiceVannucci groups.ConclusionsA hypoxic brain damage model of neonatal rat was successfully prepared using 7 minutes 0% oxygen exposure. The new approach appears to be simple and reliable.