川崎病患儿血清中hs-CRP与基质金属蛋白酶及抑制剂联合检测的临床意义

Roles of matrix metalloproteinases, tissue inhibitor of matrix metalloproteinase and hs-CRP in Kawasaki disease

摘要:

目的：检测基质金属蛋白酶(MMP)-2、MMP-9、基质金属蛋白酶抑制剂(TIMP)-1及hs-CRP在川崎病患儿血清中的表达，并探讨其与冠状动脉损伤的关系。方法：选取151例川崎病患儿作为观察组（无冠状动脉损伤40例，有冠状动脉损伤111例），60例健康儿童作为对照组，采用酶联免疫吸附实验(ELISA)检测血清中MMP-2、MMP-9和TIMP-1含量，应用终点散射比浊法检测血清hs-CRP的含量。结果：MMP-2，MMP-9及hs-CRP含量在冠脉损伤组及无冠脉损伤组与对照组比较差异均有显著性，在冠脉损伤组量最高（P<0.05）；川崎病患儿中MMP-2、MMP-9及TIMP-1之间的检测量呈正相关（P<0.05）。结论：MMP-2、MMP-9、TIMP-1及hs-CRP在川崎病的发生发展中可能起重要作用，MMP-2、MMP-9及hs-CRP的联合检测可能对判断病变程度有重要帮助。［中国当代儿科杂志，2009，11（12）：989-991］

Abstract:

OBJECTIVE: To examine the expression of matrix metalloproteinases (MMP)-2, -9, tissue inhibitor of matrix metalloproteinase (TIMP)-1 and hs-CRP, and their relationship with coronary artery in children with Kawasaki disease. METHODS: One hundred and fifty-one children with Kawasaki disease (111 cases with coronary artery damage and 40 cases without) and 60 healthy children were enrolled. The expression of MMP-2, MMP-9 and TIMP-1 was detected using ELISA, and the hs-CRP concentration was measured using the endpoint nephelometry. RESULTS: There were significant differences in the level of MMP-2, MMP-9 and hs-CRP between the patients with or without coronary artery damage and the healthy children (P<0.05). The levels of MMP-2, MMP-9 and hs-CRP were the highest in the cardiovascular damage group (P<0.05). There were positive correlations between MMP-2, MMP-9 and TIMP-1 in children with Kawasaki disease. CONCLUSIONS: MMP-2, MMP-9, TIMP-1 and hs-CRP may play important roles in the development of Kawasaki disease. The combined measurement of MMP-2, MMP-9 and hs-CRP may be useful in the evaluation of the severity in children with Kawasaki disease.［Chin J Contemp Pediatr, 2009, 11 (12):989-991］