急性特发性血小板减少性紫癜儿童T-bet和GATA3的表达

Expression of T-bet and GATA3 in children with acute idiopathic thrombocytopenic purpura

摘要:

目的：通过分析急性特发性血小板减少性紫癜（ITP）儿童血中转录因子T-bet和GATA3 mRNA的表达及细胞因子IFN-γ和IL-4的含量，探讨急性ITP发病机制中Th1/Th2细胞分化的趋势及其与转录因子的关系。方法：利用RT-PCR技术，测定急性TIP患儿发作期（n=30）和缓解期（n=28）外周血中T-bet和GATA3 mRNA 的表达，并使用ELISA法检测血浆IFN-γ和IL-4含量。20例健康儿童作为对照组。结果：ITP患儿发作期血中T-bet mRNA表达和IFN-γ含量明显高于缓解期和对照组（P＜0.01）。发作期血中GATA3 mRNA表达和IL-4的含量明显低于缓解期和对照组（P＜0.01）。结论：儿童急性ITP存在明显的Th1优势分化现象，且可能与转录因子T-bet和GATA3的调控作用有关。［中国当代儿科杂志，2010，12（1）：29-31］

Abstract:

OBJECTIVE: To study the expression of transcriptional factors T-bet and GATA3 mRNA and the levels of IFN-γ and IL-4 in blood in children with acute idiopathic thrombocytopenic purpura (ITP) and investigate the tendency of polarization of Th1/Th2 in children with ITP. METHODS: Blood T-bet and GATA3 mRNA expression were examined using RT-PCR and plasma IFN-γ and IL-4 levels were measured using EIASA in children with acute ITP in acute (n=30) and remission stages (n=28). Twenty healthy children served as the controls. RESULTS: Blood T-bet mRNA expression and IFN-γ levels in children with ITP in the acute stage were markedly higher than those in the remission stage and controls (P<0.01). In contrast, blood GATA3 mRNA expression and IL-4 levels in children with ITP in the acute stage were significantly lower than those in the remmission stage and controls (P<0.01). CONCLUSIONS: The high expression of T-bet and IFN-γ and the low expression of GATA3 and IL-4 indicate the existence of Th1 polarization in children with acute ITP. This might be related to the regulation of T-bet and GATA3.［Chin J Contemp Pediatr, 2010, 12 (1):29-31］