氯胺酮合用盐酸戊乙奎醚对新生大鼠突触素表达的影响

Effect of ketamine combined with penehyclidine hydrochloride on the expression of synaptophysin in the brain of neonatal rats

摘要:

目的：观察氯胺酮合用盐酸戊乙奎醚对新生大鼠学习记忆和海马CA3区突触素表达的影响。方法：7 d龄Sprague-Dawly大鼠80只，随机分为4组，分别腹腔注射氯胺酮50 mg/kg（K组），盐酸戊乙奎醚2 mg/kg（P组），氯胺酮50 mg/kg+盐酸戊乙奎醚2 mg/kg（PK组）及与PK组相同容积的生理盐水（NS组）。于给药后14 d 行Morris水迷宫训练；给药后24 h、14 d（水迷宫训练前）和28 d（水迷宫训练后）行免疫组化测定海马CA3区突触素的表达。结果：①水迷宫训练合格率以NS组和P组最高，PK组最低；②给药后24 h突触素表达以NS最高，依次为P组、K组及PK组（P<0.05）；给药后14 d时各组大鼠突触素表达均有增加，但NS组和P组较快，K组和PK组较慢（P<0.05）；给药后28 d时各组大鼠突触素表达差异无显著意义（P>0.05）。结论：氯胺酮与盐酸戊乙奎醚合用比单用氯胺酮更能抑制新生大鼠空间学习记忆能力和海马CA3区突触素的表达，单用盐酸戊乙奎醚无显著影响。随着年龄的增长以及适当的学习训练，突触素表达可增加至正常水平。［中国当代儿科杂志，2010，12（1）：51-55］

Abstract:

OBJECTIVE: To study the effects of ketamine combined with penehyclidine hydrochloride on the learning and memory abilities and the expression of synaptophysin in the hippocampus CA3 region in the brain of neonatal rats. METHODS: Eighty seven-day-old Sprague-Dawly rats were randomly intraperitoneally injected with 50 mg/kg of ketamine (K group), 2 mg/kg of penehyclidine hydrochloride (P group), 50 mg/kg of ketamine plus 2 mg/kg penehyclidine hydrochloride (PK group) or normal saline (control group). The rats were trained and tested in a Morris water maze 14 days after administration. The immunhistochemical method was used to ascertain the expression of synaptophysin in the hippocampus CA3 region 24 hrs, 14 days and 28 days after administration. RESULTS: In the Morris water maze training, the rats in the PK group performed worst, followed by the K group. The rats from the P and NS groups performed well. Compared with the NS group, the expression of synaptophysin in the K and the PK groups decreased significantly 24 hrs and 14 days after administration (P<0.05). The PK group had lower synaptophysin expression than the K group 24 hrs and 14 days after administration (P<0.05). Up to 28 days after administration, the synaptophysin expression increased in all of the four groups and there were no significant differences between groups. CONCLUSIONS: Ketamine combined with penehyclidine hydrochloride may inhibit more significantly learning and memory abilities and the synaptophysin expression in the hippocampus CA3 region than ketamine alone in neonatal rats. Penehyclidine hydrochloride alone has no effect on learning and memory abilities and the synaptophysin expression. The synaptophysin expression may increase to a normal level by training and with increasing age.［Chin J Contemp Pediatr, 2010, 12 (1):51-55］