幽门螺杆菌感染儿童胃黏膜细胞凋亡相关蛋白的研究

Expression of apoptosis-related proteins in gastric mucosa of children with Helicobacter pylori infection

摘要:

目的：旨在研究儿童胃黏膜细胞凋亡相关蛋白p53和Bax的表达水平与幽门螺杆菌（Hp）感染的关系。方法：采用免疫组化法检测了33例胃黏膜病变儿童胃黏膜上皮细胞中p53和Bax表达水平，并采用快速尿素酶试验和组织病理学检测两种方法检查这些病例的Hp感染情况。结果：在17例Hp阳性组织标本中，15例（88%）p53表达阳性，而在16例Hp阴性组织标本中，9例（56%）呈阳性。分析Bax的表达水平发现，在17例Hp阳性组织标本中，13例（76%）标本Bax表达呈阳性，而在16例Hp阴性组织标本中，6例 （38%）呈阳性。统计学分析显示，Hp阳性标本的p53和Bax表达水平要明显高于Hp阴性标本（P<0.05）。结论：儿童期Hp感染与胃黏膜上皮细胞p53蛋白和Bax蛋白过度表达密切相关。［中国当代儿科杂志，2010，12（2）：110-112］

Abstract:

OBJECTIVE: To investigate the relationship between apoptosis-related proteins in gastric mucosa, p53 and Bax, and Helicobacter pylori (H. pylori) infection in children. METHODS: p53 and Bax expression in gastric mucosa were measured using immunohistochemical technique in 33 children with gastric mucosal lesions. Presence/absence of H. pylori infection was detected by the rapid urease and pathological tests. RESULTS: Fifteen children (88%) showed positive expression of p53 in 17 children who were confirmed with H. pylori infection, compared with 9 (56%) in 16 H. pylori negative children. Thirteen children (76%) showed positive expression of Bax in the 17 children with H. pylori infection, compared with 6 (38%) in the 16 H. pylori negative children. The expression levels of p53 and Bax in the H. pylori positive group were significantly higher than those in the H. pylori negative group (P<0.05). CONCLUSIONS: H. pylori infection is associated with the over-expression of p53 and Bax proteins in gastric mucosa in children.［Chin J Contemp Pediatr, 2010, 12 (2):110-112］