儿童紫癜性肾炎巨噬细胞移动抑制因子表达及意义

Renal expression of macrophage migration inhibitory factor in children with Henoch-Sch-nlein purpura nephritis

摘要:

目的：探讨巨噬细胞移动抑制因子（MIF）在紫癜性肾炎（HSPN）患儿中的表达变化及意义。方法：应用免疫组织化学法检测不同程度病理损害HSPN组和对照组（薄基底膜病）肾组织标本中MIF的表达，并分析与24 h尿蛋白定量的相关关系。结果：对照组肾脏仅有微弱的MIF表达，HSPN组的MIF表达增加，且随病理分级的加重而增强。HSPN病理Ⅲ～Ⅳ级组、Ⅰ～Ⅱ级组的肾活检组织MIF阳性强度均较对照组显著增强（P<0.01）；Ⅲ～Ⅳ级组的肾活检组织MIF阳性强度较Ⅰ～Ⅱ级组显著增强（P<0.01）。在伴有新月体、炎性细胞浸润的肾组织中MIF表达增加明显。HSPN肾组织MIF表达与24 h尿蛋白定量具有显著相关性（P<0.01）。结论：MIF在介导HSPN肾损害中可能起着重要作用，其表达上调可作为反映HSPN肾病理损害程度的指标。［中国当代儿科杂志，2010，12（2）：120-122］

Abstract:

OBJECTIVE: To examine the expression of macrophage migration inhibitory factor (MIF) in renal tissues obtained from children with Henoch-Sch-nlein purpura nephritis (HSPN). METHODS: The renal tissue samples were obtained from 11 children with different pathological grades of HSPN and 8 children with thin glomerular basement membrane disease (controls). The MIF expression was measured by immunohistochemistry. The correlation between MIF expression and 24 hrs urinary protein excretions was evaluated using a linear correlation analysis. RESULTS: MIF expression was seldom found in renal tissues obtained from controls. However, a significantly increased MIF expression was found and was concordant with the increased severity of renal pathology in renal tissues obtained from children with HSPN. The MIF expression in renal tissues of grade Ⅲ-Ⅳ of renal pathology was significantly higher than that in gradeⅠ-Ⅱ in children with HSPN (P<0.01). In children with HSPN, there was an increased MIF expression in renal tissues with crescent formation and inflammatory cell infiltration. Renal MIF expression was significantly positively correlated with 24 hrs urinary protein excretions in children with HSPN (P<0.01). CONCLUSIONS: MIF may play an important role in renal injury of HSPN. Up-regulation of MIF expression may reflect the degree of renal lesions in HSPN.［Chin J Contemp Pediatr, 2010, 12 (2):120-122］