毛细支气管炎患儿ICAM-1和RANTES检测的意义

Detection of ICAM-1 and RANTES in infants with bronchiolitis

摘要:

目的：探讨毛细支气管炎（简称毛支炎）患儿细胞间黏附分子-1（ICAM-1）和激活调节正常T细胞表达和分泌因子（RANTES）检测的意义及相关性。方法：采用流式细胞术检测28例毛支炎患儿、23例支气管肺炎患儿及24例健康婴儿外周血淋巴细胞ICAM-1表达情况，ELISA法测定其血清RANTES水平，并进行相关性分析。结果：毛支炎组ICAM-1表达(35.0±10.3%)高于肺炎组（29.9±8.6%; P<0.05）及对照组（24.6±6.9%; P<0.01），肺炎组ICAM-1表达高于对照组（P<0.05）。毛支炎组和肺炎组RANTES水平分别为32.1±6.0和 30.6±6.2 ng/mL，高于对照组的27.1±5.1 ng/mL（分别P<0.01，<0.05），毛支炎组与肺炎组差异无统计学意义。ICAM-1与RANTES在毛支炎组呈正相关（r=0.675，P<0.01）。结论：ICAM-1和 RANTES参与了毛支炎的发病过程且具有协同作用。［中国当代儿科杂志，2010，12（3）：181-183］

Abstract:

OBJECTIVE: To explore the role of intercellular adhesion molecule-1 (ICAM-1) and regulated upon activation normal T cell expressed and secreted (RANTES) in bronchiolitis and their correlation in the pathogenesis of this disorder. METHODS: The expression of ICAM-1 was detected by flow cytometry on lymphocytes of peripheral blood in 28 infants with bronchiolitis, 23 infants with bronchopneumonia and 24 healthy infants. Serum level of RANTES was assayed using ELISA. The correlation between ICAM-1 and RANTES levels was evaluated using Pearson correlation coefficient. RESULTS: The ICAM-1 level in the bronchiolitis group (35.0±10.3%) was much higher than that in the bronchopneumonia (29.9±8.6%; P<0.05) and the control groups (24.6±6.9%; P<0.01). The bronchopneumonia group had higher ICAM-1 level than the control group (P<0.05). The RANTES level in the bronchiolitis (32.1±6.0 ng/mL) and the bronchopneumonia groups (30.6±6.2 ng/mL) was significantly higher than that in the control group (27.1±5.1 ng/mL) (P<0.01, P<0.05, respectively), however, no significant difference was found between the bronchopneumonia and bronchiolitis groups. There was a positive correlation between ICAM-1 and RANTES levels in the bronchiolitis group (r=0.675, P＜0.01). CONCLUSIONS: ICAM-1 and RANTES are involved in the pathogenesis of bronchiolitis and show a synergistic effect.［Chin J Contemp Pediatr, 2010, 12 (3):181-183］