SDF-1及CXCR4在哮喘小鼠肺组织中的表达及布地奈德的干预作用

Expression of stromal cell derived factor-1 and CXC chemokine receptor 4 and the effects of budesonide on their expression in mice with asthma

摘要:

目的：探讨哮喘小鼠发病过程中基质细胞衍生因子-1（SDF-1）及其受体CXCR4在肺内表达的变化及布地奈德的干预影响。方法：清洁级雄性BALB/c小鼠30只，随机分为对照组、干预组及哮喘组。观察卵蛋白激发及用布地奈德干预后哮喘小鼠气道的病理变化；免疫组织化学染色观察SDF-1表达的变化；RT-PCR法观察CXCR4 mRNA表达的变化。结果：SDF-1及CXCR4 mRNA在对照组中呈低表达，在哮喘组中表达明显增加，使用布地奈德进行干预后SDF-1及CXCR4 mRNA的表达明显降低（0.426±0.052 vs 0.361±0.065；0.829±0.027 vs 0.723±0.094; P<0.05），且二者表达量均与气道壁厚度呈正相关（r=0.744，P<0.01；r=0.553, P<0.01）。结论：SDF-1及其受体CXCR4可能参与了哮喘小鼠气道重塑过程，布地奈德干预改善哮喘小鼠的气道重塑可能与降低 SDF-1及CXCR的表达有关。［中国当代儿科杂志，2010，12（3）：215-218］

Abstract:

OBJECTIVE: To study the expression of stromal cell derived factor-1(SDF-1) and CXC chemokine receptor 4 (CXCR4) in the airway and the effect of budesonide on their expression in mice with asthma. METHODS: Thirty BALB/c male mices were randomly divided into three groups: placebo control, untreated asthma, and budesonide-treated asthma. The asthma group were induced by intraperitoneal injection of 10% ovalbumin (OVA ) on days 1, 8 and 15, and then from days 22 to 34, challenged by inhalation of 2% OVA aerosol every other day. The budesonide-treated asthma group received an inhalation of budesonide (1 mg ) before OVA challenge. The pathological changes of the airway were assessed by hematoxylin and eosin staining. The immunohistochemistry was used to estimate the expression of SDF-1 in the lung. RT-PCR was used to evaluate the expression of CXCR4 in the lung. RESULTS: Compared with the control group, SDF-1 and CXCR4 expression in the lung in the untreated asthma group increased significantly (P<0.05). The budesonide-treated asthma group demonstrated significantly decreased SDF-1 (0.426±0.052 vs 0.361±0.065; P<0.05) and CXCR4 (0.829±0.027 vs 0.723±0.094; P<0.05) expression in the lung as compared with the untreated asthma group. Both SDF-1 (r=0.744, P<0.01) and CXCR4 (r=0.553, P<0.01）were positively correlated with the thickness of the airway wall. CONCLUSIONS: SDF-1 and CXCR4 may be associated with airway remodeling in mice with asthma. Budesonide can improve airway remodeling, possibly by decreasing the expression of SDF-1 and CXCR4.［Chin J Contemp Pediatr, 2010, 12 (3):215-218］