黄芪甲甙对骨髓间充质干细胞分泌干细胞因子的影响

Stem cell factor secretion by bone mesenchymal stem cells stimulated with astragaloside IV

摘要:

目的：初步探讨中药黄芪甲甙对大鼠骨髓间充质干细胞（MSCs）表达细胞因子的影响。方法：全骨髓贴壁法和单克隆培养法分离培养大鼠MSCs，间接免疫荧光法鉴定其生物学特性，用MTT法检测黄芪甲甙对MSCs增殖的影响，RT-PCR方法检测多种细胞因子的表达及黄芪甲甙对细胞因子的影响。结果：成功分离出大鼠MSCs，有干细胞因子（SCF）、血小板生成素（TPO）、粒细胞巨噬细胞集落刺激因子（GM-CSF）、转化生长因子（TGF-β1）的表达，不表达白介素-3（IL-3）；加入不同浓度的黄芪甲甙与MSCs共培养24、48、72 h后，以200 mg/mL黄芪甲甙组72 h增殖作用最明显（P<0.05）；RT-PCR分析示黄芪甲甙培养组MSCs分泌SCF mRNA高于未加黄芪甲甙的对照组（P<0.01），其余细胞因子表达量与对照组相比差异无统计学意义。结论：黄芪甲甙能促进MSCs体外增殖，并可以诱导和促进MSCs分泌SCF，这可能揭示了其促进MSCs增殖和分化的部分机制。［中国当代儿科杂志，2010，12（4）：290-292］

Abstract:

OBJECTIVE: To study the effect of astragaloside IV on the expression of cytokines in bone mesenchymal stem cells (MSCs) in rats. METHODS: MSCs were isolated from Wistar rats by the method of adhesive cultiration and clone, and then their biological activities were assessed using indirect immunofluorescence. Proliferation of MSCs stimulated with astragaloside IV was ascertained by the MTT method. Expression of cytokines was ascertained using RT-PCR in MSCs with astragaloside IV stimulation or not. RESULTS: MSCs were effectively isolated and purified in vitro, and had expression of many cytokines except IL-3, such as stem cell factor (SCF), thrombopoietin (TPO), granulocyte macrophage colony stimulating factor (GM-CSF) and transforming growth factor (TGF-β1). Astragaloside IV stimulation promoted MSCs proliferation, and 200 mg/mL astragaloside IV treatment produced a peak effect 72 hrs after culture. The SCF expression in MSCs stimulated with astragaloside IV increased significantly compared with that in MSCs without astragaloside IV stimulation. CONCLUSIONS: Astragaloside IV may promote MSCs proliferation and increase SCF secretion in vitro.［Chin J Contemp Pediatr, 2010, 12 (4):290-292］