缺氧缺血性脑损伤新生大鼠脑组织小RNA表达的变化

Expression of microRNA in neonatal rats with hypoxic-ischemic brain damage

摘要:

目的：观察新生大鼠缺氧缺血性脑损伤（HIBD）皮层小RNA（microRNA，miRNA）差异表达，以研究其在HIBD的病理生理中的作用。方法：建立新生大鼠HIBD模型14 d后，取皮层脑组织，miRNA表达谱芯片检测miRNA差异表达。实时定量PCR检测miR-126-26a、-674-5p、-21、-25、-290和miR-124、-125b-5p、-9a等9个miRNA。结果：miRNA表达谱芯片结果提示，与对照组比较，HIBD大鼠皮层脑组织中27个已知miRNA表达上调2倍以上；60个表达下调2倍以上。实时定量PCR检测所选择的9个miRNA结果与miRNA芯片结果具有同样趋势。结论：HIBD模型大鼠miRNA表达有明显差异，这些改变可能在HIBD的病理生理中起着重要作用。［中国当代儿科杂志，2010，12（5）：373-376］

Abstract:

OBJECTIVE: To study the changes of microRNA expression in cortex tissues in neonatal rats with hypoxic-ischemic brain damage（HIBD）and the possible roles of microRNA in the pathogenesis of HIBD.MethodsRat HIBD model was prepared. The cortex tissues were obtained 14 days after the HIBD event. The microRNA expression profiles were measured using microRNA microarray. Expression of 9 microRNAs (miR-126,-26a,-674-5p,-21,-25,-290, miR-124,-125b-5p and microRNA-9a) was determined by quantitative real-time PCR. RESULTS: he results of microRNA expression profiles indicated that 27 pieces of microRNA were up-regulated more than 2 folds and 60 pieces were down-regulated more than 2 folds compared with the normal control group. The results of the 9 microRNAs detected by quantitative real-time PCR were consistent with those detected by microRNA microarray. CONCLUSIONS: HIBD rats have significant changes in microRNA expression, suggesting that microRNA expression may play important roles in the pathogenesis of HIBD.［Chin J Contemp Pediatr, 2010, 12 (5):373-376］