川崎病患儿循环内皮祖细胞功能与血浆超敏C反应蛋白浓度的关系

Decreased circulating endothelial progenitor cell function: relationship with serum concentrations of high-sensitivity C-reactive protein in children with Kawasaki disease

摘要:

目的：研究川崎病患儿循环内皮祖细胞的功能状态及其与血浆超敏C反应蛋白（Hs-CRP）浓度的关系。方法：将10例川崎病患儿和10例健康体检儿童分为川崎病组和对照组。利用含有血管内皮细胞生长因子（VEGF），碱性成纤维细胞生长因子(bFGF)的DMEM培养基将入选者的外周血单个核细胞诱导培养为内皮祖细胞。然后分别利用MTT法，改良Boyden小室法及细胞培养板贴壁法测定内皮祖细胞的增殖、迁移和贴壁功能。利用胶乳增强的免疫比浊法测定血浆Hs-CRP的浓度。结果：川崎病组内皮祖细胞的增殖能力、迁移能力和贴壁能力均较对照组显著降低，差异有统计学意义，P<0.01。川崎病组Hs-CRP的浓度较对照组显著升高(87.1±30.2 mg/L vs 5.3±3.4 mg/L;P<0.01)。川崎病组循环内皮祖细胞功能与血浆Hs-CRP浓度呈显著负相关。结论：川崎病患儿循环内皮祖细胞功能显著降低，这可能与川崎病患儿体内的炎症介质反应有关。［中国当代儿科杂志，2010，12（7）：513-517］

Abstract:

OBJECTIVE: To study the function of circulating endothelial progenitor cells and its relationship with serum concentrations of high-sensitivity C-reactive protein (Hs-CRP) in children with Kawasaki disease. METHODS: Ten children with Kawasaki disease and ten healthy children as a control group were enrolled. The peripheral mononuclear cells were induced into endothelial progenitor cells using Dulbecco’s Modified Eagle Medium containing vascular endothelial growth factor and basic fibroblast growth factor. The proliferative ability, migratory ability and adhesive ability of endothelial progenitor cells were assessed by MTT methods, modified Boyden chamber methods and cell culture plate adhesion method, respectively. The concentrations of serum Hs-CRP were measured by latex enhanced turbidimetric immunoassay. RESULTS: The proliferative ability, migratory ability and adhesive ability of endothelial progenitor cells in the Kawasaki disease group were significantly lower than those in the control group (P<0.01). The serum concentrations of Hs-CRP in the Kawasaki disease group were significantly higher than those in the control group (87.1±30.2 mg/L vs 5.3±3.4 mg/L; P<0.01). The function of circulating endothelial progenitor cells was negatively correlated with serum concentrations of Hs-CRP in the Kawasaki disease group. CONCLUSIONS: The function of circulating endothelial progenitor cells is decreased in children with Kawasaki disease, which may be associated with the abnormal expression of inflammatory mediators.［Chin J Contemp Pediatr, 2010, 12 (7):513-517］