川崎病冠状动脉损伤与纤维蛋白原Bβ-148C/T基因多态性的关系

Relationship between fibrinogen Bβ-148C/T polymorphism and coronary artery lesions in children with Kawasaki disease

摘要:

目的：探讨纤维蛋白原Bβ-148C/T基因多态性与川崎病冠状动脉损伤的关系。方法：收集36例川崎病患儿与49例健康对照儿童的空腹静脉血，用辅助血浆纤维蛋白原活性测定系统测定纤维蛋白原水平和纤维蛋白原的分子功能；采用多聚酶链反应,限制性酶切方法对纤维蛋白原Bβ-148C/T位点的基因型进行测定。结果：川崎病儿童冠状动脉扩张组血浆纤维蛋白原水平明显高于非扩张组及健康对照组(P<0.01); 川崎病儿童冠状动脉扩张组T等位基因频率明显高于健康对照组及非扩张组 (P<0.01)。结论：纤维蛋白原β-148基因多态性及血浆纤维蛋白原水平与川崎病患儿冠状动脉扩张有相关性。［中国当代儿科杂志，2010，12（7）：518-520］

Abstract:

OBJECTIVE: To study the possible relationship between coronary artery lesions and fibrinogen Bβ-148 C/T polymorphism in children with Kawasaki disease. METHODS: Fast blood samples were taken from 36 children with Kawasaki disease (21 had coronary artery lesions) and 49 age-and gender-matched healthy children (control group). Plasma levels and molecular reactivity of fibrinogen were measured with Assist Plasma Fibrinogen Activity Assay System. Polymerize chain reaction and restriction enzyme digestion were used to detect the genotypes of fibrinogen Bβ-148C/T gene polymorphism. RESULTS: The plasma fibrinogen levels in patients with coronary artery lesions were significantly higher than those in patients without coronary artery lesions and in the control group. T allele frequency in patients with Kawasaki disease was significantly higher than that in the control group. The patients with coronary artery lesions had more increased T allele frequency compared with the patients without coronary artery lesions. CONCLUSIONS: Plasma fibrinogen levels and fibrinogen Bβ-148C/T polymorphism are associated with coronary artery lesions in children with Kawasaki disease.［Chin J Contemp Pediatr, 2010, 12 (7):518-520］