IL-27对哮喘患儿IFN-γ及IL-4的调节作用

Interleukin-27 affects IFN-γ and IL-4 activities in children with asthma

摘要:

目的：探讨白介素27（IL-27）对哮喘患儿干扰素γ（IFN-γ）及白介素4（IL-4）的调节作用。方法：收集23例哮喘患儿外周血单个核细胞（PBMC），加入不同浓度（5 ng/mL或0.5 ng/mL）重组人白介素27（rh IL-27）进行体外培养12 h，予酶联免疫吸附法（ELISA）测定其培养上清IFN-γ及IL-4浓度。结果：5 ng/mL和0.5 ng/mL两个浓度的rh IL-27刺激PBMC进行体外培养后，IFN-γ表达分别为85.9±12.2和8.9±2.3 μg/L，较细胞刺激素组(7.2±1.3 μg/L)均有显著升高（P＜0.01或0.05）。5 ng/mL rh IL-27组IL-4表达较细胞刺激素组有明显降低（15.0±1.9 μg/L vs 77.0±15.6 μg/L,P＜0.01）。结论：IL-27参与了哮喘的发病过程，它可通过调节Th1/Th2相关细胞因子活性为哮喘治疗提供一种新的途径。［中国当代儿科杂志，2010，12（7）：521-523］

Abstract:

OBJECTIVE: To study the effect of interleukin (IL)-27 on INF and IL-4 activities in children with asthma. METHODS: The levels of IFN-γ and IL-4 in culture supernatants of peripheral blood mononuclear cells (PBMCs) from 23 asthmatic children were measured using ELISA. PBMCs were cultured with recombinant IL-27（5 ng/mL or 0.5 ng/mL）for 12 hrs in vitro. RESULTS: IFN-γ levels in both 5 ng/mL and 0.5 ng/mL IL-27 treatment groups (85.9±12.2 and 8.9±2.3 μg/L, respectively) increased compared with those in the cytokine stimulated control group (P＜0.01, P＜0.05 respectively). The group treated with 5 ng/mL IL-27 had decreased significantly IL-4 levels compared with the cytokine stimulated control group (15.0±1.9 μg/L vs 77.0±15.6 μg/L; P＜0.01). CONCLUSIONS: IL-27 may be involved in the pathogenesis of asthma. It may affect Th1/Th2 cell activities and might be a new option for the treatment of asthma.［Chin J Contemp Pediatr, 2010, 12 (7):521-523］